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El Altiplano: ciencia y conciencia en los Andes
(Universidad de Chile, 1997)
Briones M.
EL ALTIPLANO ECUATORIAL ANDINO, ESTUDIOS SOBRE SU ESPECIFICAD CULTURAL
Segundo E. Moreno Yañez
AREA BIOMEDICINA Y FISIOLOGIA DE ALTURA
BASES BIOLOGICAS DEL MAL DE MONTAÑA CRONICO.
Carlos Monge C. y María del Pilar Fortunic
¿ ES EVITABLE LA...
.000 m sobre el nivel del mar. Se ubica en los Andes Centrales y es un territorio compartido por Argentina, Bolivia, Chile y Perú. Su población principalmente aymara y quechua mantiene estrechos lazos con su cultura prehispánica y depende...
.000 m sobre el nivel del mar. Se ubica en los Andes Centrales y es un territorio compartido por Argentina, Bolivia, Chile y Perú. Su población principalmente aymara y quechua mantiene estrechos lazos con su cultura prehispánica y depende...
Creación desde la tragedia musical : Jorge Matute Johns (ópera)
(Universidad de Chile, 2017)
La presente tesis procura argumentar el acto creativo, realizado por medio del lenguaje musical escrito, de nuestra ópera inspirada en el Caso Matute. Para tales efectos debemos conocer, antes que todo, los fundamentos que nos puedan ayudar a...
Vesículas extracelulares de origen cerebral aisladas desde suero presentan perfil proteico diferencial en dos modelos de estrés en ratas
(Universidad de Chile, 2020)
El estrés precipita un espectro de trastornos neuropsiquiátricos, que incluyen trastornos del estado de ánimo, es decir, entidades complejas y multifactoriales caracterizadas por una gama de síntomas presentes en diferentes combinaciones, lo que...
Stress precipitates a spectrum of neuropsychiatric disorders, including mood disorders, that is, complex and multifactorial entities characterized by a range of symptoms present in different combinations, suggesting the existence of stress-triggered disease subtypes. Using animal models, in our laboratory it was previously described that repetitive stress through two protocols: movement restriction, or immobilization, induced depressive-type behaviors in rats that were differentially reversed by antidepressant drugs that raise serotonin or norepinephrine levels, respectively. This suggests that the neurobiological mechanisms activated by both stressors are different. To characterize both stress protocols, we proposed to identify protein biomarkers in cerebrospinal fluid, finding that Aldolase C, an enzyme expressed in astrocytes, is present at differential levels in nanovesicular structures. Extracellular nanovesicles (ENVs) are secreted by all cell types, and they contain from proteins to miRNAs, and growing evidence points to their role in extracellular communication. The possibility that ENVs of brain origin could contain possible stress biomarkers would facilitate the diagnosis of complex psychiatric disorders. Therefore, we postulate the following hypothesis: ENVs of brain origin, obtained from serum, contain a differential protein profile in two models of chronic stress. To test our hypothesis, serum ENVs from rats exposed to stress were characterized, and their proteomes were identified by mass spectrometry. When validating the results, it was observed that Adolase C and the glial fibrillar acid astrocytic protein (GFAP) show differential levels in ENVs when comparing restriction with immobilization. To check whether Aldolase C in serum ENVs could be derived from forebrain cells, we transferred Aldolase C-GFP to astrocytes by in utero electroporation. Recombinant protein was recovered in serum ENVs, providing direct evidence that brain-derived ENVs are present in serum, while their molecular load could be a valuable source of biomarkers for central nervous system diseases...
Stress precipitates a spectrum of neuropsychiatric disorders, including mood disorders, that is, complex and multifactorial entities characterized by a range of symptoms present in different combinations, suggesting the existence of stress-triggered disease subtypes. Using animal models, in our laboratory it was previously described that repetitive stress through two protocols: movement restriction, or immobilization, induced depressive-type behaviors in rats that were differentially reversed by antidepressant drugs that raise serotonin or norepinephrine levels, respectively. This suggests that the neurobiological mechanisms activated by both stressors are different. To characterize both stress protocols, we proposed to identify protein biomarkers in cerebrospinal fluid, finding that Aldolase C, an enzyme expressed in astrocytes, is present at differential levels in nanovesicular structures. Extracellular nanovesicles (ENVs) are secreted by all cell types, and they contain from proteins to miRNAs, and growing evidence points to their role in extracellular communication. The possibility that ENVs of brain origin could contain possible stress biomarkers would facilitate the diagnosis of complex psychiatric disorders. Therefore, we postulate the following hypothesis: ENVs of brain origin, obtained from serum, contain a differential protein profile in two models of chronic stress. To test our hypothesis, serum ENVs from rats exposed to stress were characterized, and their proteomes were identified by mass spectrometry. When validating the results, it was observed that Adolase C and the glial fibrillar acid astrocytic protein (GFAP) show differential levels in ENVs when comparing restriction with immobilization. To check whether Aldolase C in serum ENVs could be derived from forebrain cells, we transferred Aldolase C-GFP to astrocytes by in utero electroporation. Recombinant protein was recovered in serum ENVs, providing direct evidence that brain-derived ENVs are present in serum, while their molecular load could be a valuable source of biomarkers for central nervous system diseases...
Plan de Negocios para la Exportación de Eucaliptus al Mercado Chino
(Universidad de ChilePrograma Cybertesis, 2009)
El objetivo de la presente Tesis consiste en el desarrollo de un Plan de Negocios, que permita evaluar la factibilidad de elaborar astillas de eucaliptus y comercializarlas en el mercado Chino, integrando verticalmente hacia delante a una...