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Desarrollo de turismo científico en Cerro Galera, transformación territorial a través del conocimiento local y científico, ruralidad de Aysén
(Universidad de Chile, 2012)
CIENTÍFICO. RURALIDAD DE AYSÉN.
Memoria para optar al título de Geógrafa.
JACQUELINE BOLDT CORVALÁN
Profesor Guía: ENRIQUE ALISTE ALMUNA
SANTIAGO- CHILE
Agosto 2012
Jacqueline Boldt Corvalán...
” para el Análisis de Información��...�...�...16 4.2.-Herramientas Metodológicas�������������������...���...�18 4.2.1.- Entrevistas Semiestructuradas��...�������������.��....18 4.2.2.- Mapa Mental�������������������������..�..18 4.2.3.- Cartografía...
” para el Análisis de Información��...�...�...16 4.2.-Herramientas Metodológicas�������������������...���...�18 4.2.1.- Entrevistas Semiestructuradas��...�������������.��....18 4.2.2.- Mapa Mental�������������������������..�..18 4.2.3.- Cartografía...
Implicancias y alcances de la Ley no. 20.609 contra la discriminación arbitraria, en materia laboral : análisis jurisprudencial comparativo en relación con el procedimiento de tutela laboral
(Universidad de Chile, 2017)
IMPLICANCIAS Y ALCANCES DE LA LEY N°20.609 CONTRA LA
DISCRIMINACIÓN ARBITRARIA, EN MATERIA LABORAL.
Análisis jurisprudencial comparativo en relación con el Procedimiento de
Tutela Laboral.
Memoria para optar al grado de...
seguir compartiendo juntas este loco proceso que se llama vida. A las amigas de la vida, Bárbara, Beatriz y Tatiana por alentarme en este proceso y siempre darme aliento para seguir. Y finalmente, pero no menos importante, a Monika y Albert, por ser los...
seguir compartiendo juntas este loco proceso que se llama vida. A las amigas de la vida, Bárbara, Beatriz y Tatiana por alentarme en este proceso y siempre darme aliento para seguir. Y finalmente, pero no menos importante, a Monika y Albert, por ser los...
Caracterización de música según emociones y complejidad, utilizando RNN-LSTM y teoría de la información, para analizar sus efectos sobre la empatía hacia el dolor
(Universidad de Chile, 2019)
La empatía en la humanidad es un elemento fundamental para construir una sociedad justa. A su vez la empatía puede ser modulada por diferentes factores, como la emoción que tiene un individuo. Por ende la música, como detonante de emociones en el...
Trayectorias residenciales y prácticas espaciales de los hogares migrantes latinoamericanos del centro y pericentro de la ciudad de Iquique
(Universidad de Chile, 2014)
DEL CENTRO Y PERICENTRO DE LA CIUDAD
DE IQUIQUE
Memoria para optar al título profesional de Geógrafo
PEDRO ANTONIO PALMA CALORIO
Proyecto FONDECYT N° 11121241
“Mercado de arriendo y trayectorias residenciales de los precarios...
IQUIQUE AGRADECIMIENTOS Agradecer siempre es insuficiente para retribuir el cariño entregado durante todos estos años de formación, pero no existe una palabra más potente, tampoco sinónimo para reemplazar las infinitas gracias que debo a mi...
IQUIQUE AGRADECIMIENTOS Agradecer siempre es insuficiente para retribuir el cariño entregado durante todos estos años de formación, pero no existe una palabra más potente, tampoco sinónimo para reemplazar las infinitas gracias que debo a mi...
Arraigo y territorialidad en habitantes de campamentos de la Región de O'Higgins
(Universidad de Chile, 2016)
1
Universidad de Chile
Facultad de Arquitectura y Urbanismo
Escuela de Postgrado
ARRAIGO Y TERRITORIALIDAD EN HABITANTES DE CAMPAMENTOS DE
LA REGIÓN DE O´HIGGINS
Tesis para optar al grado de...
........................ 59 3.2.3 Levantamiento y análisis de información cualitativa ........................... 60 3.3 ASPECTOS CUANTITATIVOS ............................................................ 62 3.3.1 Criterios para la elección del área de estudio...
........................ 59 3.2.3 Levantamiento y análisis de información cualitativa ........................... 60 3.3 ASPECTOS CUANTITATIVOS ............................................................ 62 3.3.1 Criterios para la elección del área de estudio...
f.a.s.t
(2016)
f.a.s.t.”, utilizando la tecnología EMS (Electrical Muscle Stimulation). El segmento-usuarios, corresponde a aquellas personas con interés hacia la actividad física y el cuidado personal, que disponen de poco tiempo para realizar la actividad, por lo...
Estudio de brechas de género en trayectorias de egresados del Departamento de Ingeniería Industrial de la Universidad de Chile y propuesta de políticas internas para mitigarlas
(Universidad de Chile, 2020)
lo detectado y a las 115 propuestas propias de los encuestados, para mitigar las brechas encontradas en confianza con el fin de impactar en la participación de cuerpos docentes, el acceso a cargos de mayor responsabilidad, renta bruta, entre otras...
We aukiñ zugu, historia de los medios de comunicación mapuche
(Universidad de Chile, 2011)
voz propia, un propio proyecto comunicacional que tanto intenta romper el bochornoso cerco mediático que los rodea desde hace 150 años -como puede verse en la editorial de El Mercurio antes citada- como pretende ser una voz, un camino válido para...
Diferenciación neurogénica de células madre mesenquimáticas (CMM) obtenidas desde médula ósea fetal bovina
(Universidad de Chile, 2017)
, respectivamente). Mediante citometría de flujo se determinó una alta proporción de CMM positivas para marcadores mesenquimáticos CD29 (76,3%), CD73 (96,8%) y marcadores de pluripotencia Oct4 (94,6%) y Nanog (88,4%). En contraste, una alta proporción de CMM fue...
Mesenchymal stem cells (MSC) are multipotent progenitor cells that possess the potential for self-renewal and multilineage differentiation (osteogenic, chondrogenic and adipogenic). Despite MSC have been isolated from several tissues, the most common source of isolation is the bone marrow (BM), both for clinical and research purposes. The differentiation capacity of MSC extends primarily to mesodermal lineages; however, recent studies have shown that MSC have also the potential to differentiate into ectodermal cell types such as neuronal. The aim of this study was to determine the potential for in vitro neurogenic differentiation of MSC isolated from fetal bovine BM. Detection of mesenchymal markers CD90, CD105 and CD73, hematopoietic markers CD34 and CD45 was performed by Quantitative-PCR (Q-PCR) and flow cytometry. Protocol 1 of neurogenic differentiation consisted in pre-induction for 24 hours with DMEM supplemented with 20% fetal bovine serum (FBS), and induction with beta-mercaptoethanol (BME) for 6 days. Protocol 2 of neurogenic differentiation consisted in culture of MSC in DMEM supplemented with Fibroblast Growth Factor-basic (FGFb) and Epidermal Growth Factor (EGF) for 24 hours, followed by culture in DMEM supplemented with butylated hydroxyanisole, KCl, valproic acid, forskolin, neural supplement for 120 hours. Cell samples were taken at 0, 24, 96 and 144 hours of culture. Analyses indicated that mRNA levels of mesenchymal markers CD73, CD90 and CD105 were higher (151.2, 245.1 and 238.1 fold, respectively; P <0.05) relative to CD34. Moreover, flow cytometry detected a high proportion of MSC positive for mesenchymal markers CD29 (76.3%), CD73 (96.8%), pluripotent markers Oct4 (94.6%) and Nanog (88.4%). In contrast, high proportion of MSC were negative to hematopoietic markers CD34 and CD45 (93.4% and 95.6%, respectively). During protocol 1 of neuronal differentiation, NESTIN mRNA levels decreased (P <0.05) at 24, 96 and 144 hours (3,7; 2 and 1 fold 0 hours). In contrast, levels of mRNA of MAP2 increased (P <0.05) at 24, 96 and 144 hours (2,4; 18,9 and 16 fold 0 hours). Similarly, levels of TRKA mRNA increased (P<0.05) at 96 and 144 hours (6,6 and 8 fold 0 hours). NGF and NANOG mRNA levels were not significantly different between treatments. During protocol 2, NESTIN mRNA levels decreased (P <0.05) at 24, 96 and 144 hours (3.74, 0.3, 0.1 fold 0 hours). In comparison, MAP2 mRNA levels increased (P <0.05) at 96 and 144 hours (4,1; 22,8 fold 0 hours). In addition, NGF mRNA levels were lower (P<0,05) in differentiated MSC at 0, 24, 96 and 144 hours of culture (1; 0.8; 16.2 and 17.4 fold the expression at 0 hours) compared to control (1; 5.8; 47.8 and 25.7 fold 0 hours). The gene relative expression values in differentiated MSC were consistent with immunofluorescence patterns. Although BME induced neuron-like changes in MSC morphology, gene expression profiles showed no indication of the adoption of a neurogenic phenotype. As reported before, BME-induced morphological changes may be due to cytotoxic effects rather than neurogenic induction. However, the second protocol induced morphologic changes and a gene expression pattern associated to neurogenic differentiation. In conclusion, undifferentiated MSC isolated from fetal bovine BM possess a mesenchymal profile and under adequate in vitro culture conditions may be induced into neurogenic differentiation....
Mesenchymal stem cells (MSC) are multipotent progenitor cells that possess the potential for self-renewal and multilineage differentiation (osteogenic, chondrogenic and adipogenic). Despite MSC have been isolated from several tissues, the most common source of isolation is the bone marrow (BM), both for clinical and research purposes. The differentiation capacity of MSC extends primarily to mesodermal lineages; however, recent studies have shown that MSC have also the potential to differentiate into ectodermal cell types such as neuronal. The aim of this study was to determine the potential for in vitro neurogenic differentiation of MSC isolated from fetal bovine BM. Detection of mesenchymal markers CD90, CD105 and CD73, hematopoietic markers CD34 and CD45 was performed by Quantitative-PCR (Q-PCR) and flow cytometry. Protocol 1 of neurogenic differentiation consisted in pre-induction for 24 hours with DMEM supplemented with 20% fetal bovine serum (FBS), and induction with beta-mercaptoethanol (BME) for 6 days. Protocol 2 of neurogenic differentiation consisted in culture of MSC in DMEM supplemented with Fibroblast Growth Factor-basic (FGFb) and Epidermal Growth Factor (EGF) for 24 hours, followed by culture in DMEM supplemented with butylated hydroxyanisole, KCl, valproic acid, forskolin, neural supplement for 120 hours. Cell samples were taken at 0, 24, 96 and 144 hours of culture. Analyses indicated that mRNA levels of mesenchymal markers CD73, CD90 and CD105 were higher (151.2, 245.1 and 238.1 fold, respectively; P <0.05) relative to CD34. Moreover, flow cytometry detected a high proportion of MSC positive for mesenchymal markers CD29 (76.3%), CD73 (96.8%), pluripotent markers Oct4 (94.6%) and Nanog (88.4%). In contrast, high proportion of MSC were negative to hematopoietic markers CD34 and CD45 (93.4% and 95.6%, respectively). During protocol 1 of neuronal differentiation, NESTIN mRNA levels decreased (P <0.05) at 24, 96 and 144 hours (3,7; 2 and 1 fold 0 hours). In contrast, levels of mRNA of MAP2 increased (P <0.05) at 24, 96 and 144 hours (2,4; 18,9 and 16 fold 0 hours). Similarly, levels of TRKA mRNA increased (P<0.05) at 96 and 144 hours (6,6 and 8 fold 0 hours). NGF and NANOG mRNA levels were not significantly different between treatments. During protocol 2, NESTIN mRNA levels decreased (P <0.05) at 24, 96 and 144 hours (3.74, 0.3, 0.1 fold 0 hours). In comparison, MAP2 mRNA levels increased (P <0.05) at 96 and 144 hours (4,1; 22,8 fold 0 hours). In addition, NGF mRNA levels were lower (P<0,05) in differentiated MSC at 0, 24, 96 and 144 hours of culture (1; 0.8; 16.2 and 17.4 fold the expression at 0 hours) compared to control (1; 5.8; 47.8 and 25.7 fold 0 hours). The gene relative expression values in differentiated MSC were consistent with immunofluorescence patterns. Although BME induced neuron-like changes in MSC morphology, gene expression profiles showed no indication of the adoption of a neurogenic phenotype. As reported before, BME-induced morphological changes may be due to cytotoxic effects rather than neurogenic induction. However, the second protocol induced morphologic changes and a gene expression pattern associated to neurogenic differentiation. In conclusion, undifferentiated MSC isolated from fetal bovine BM possess a mesenchymal profile and under adequate in vitro culture conditions may be induced into neurogenic differentiation....
Agua, tradición y desarrollo: análisis del conflicto entre la Comunidad Indígena Chusmiza – Usmagama y la embotelladora Chusmiza S.A.
(Universidad de Chile, 2009-09-02)
importantes cuestionamientos sobre el papel del Estado a la hora de generar los marcos adecuados para la resolución de conflictos no solo hídricos, sino ambientales en general...