Intracellular calcium and arachidonic acid increase SNAP-25 expression in cultured rat hippocampal explants, but not in cultured rat cerebellar explants
Author | dc.contributor.author | Sepúlveda, C. M. | |
Author | dc.contributor.author | Troncoso, C. C. | es_CL |
Author | dc.contributor.author | Lara Peñaloza, Hernán | es_CL |
Author | dc.contributor.author | Cárdenas, Ana María | es_CL |
Admission date | dc.date.accessioned | 2008-11-10T15:08:22Z | |
Available date | dc.date.available | 2008-11-10T15:08:22Z | |
Publication date | dc.date.issued | 1998-08-14 | |
Cita de ítem | dc.identifier.citation | NEUROSCIENCE LETTERS 252(2):127-130 | en |
Identifier | dc.identifier.issn | 0304-3940 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/120555 | |
Abstract | dc.description.abstract | The effects of the increase of intracellular calcium, induced by membrane depolarization with 50 mM KCl, and arachidonic acid (AA) on the expression of 25-kD synaptosomal-associated protein (SNAP-25) were studied in cultured rat hippocampal and cerebellar explants, and PC12 rat pheochromocytoma cells, using immunoblot analysis. Incubation periods of 24 h and 48 h in 50 mM KCl increased SNAP-25 levels in hippocampal explants and PC12 cells, but not on cerebellar explants. Otherwise, a 24 h incubation with 10 mu M AA increased SNAP-25 expression only in hippocampal explants, although 100 ng/ml phorbol 12-myristate 13-acetate (PMA) did not have effect. These results indicate that intracellular calcium and AA can modulate the expression of SNAP-25, depending on the origin of the tissue. | en |
Lenguage | dc.language.iso | en | en |
Publisher | dc.publisher | ELSEVIER SCI IRELAND LTD | en |
Keywords | dc.subject | PROTEIN-KINASE-C | en |
Título | dc.title | Intracellular calcium and arachidonic acid increase SNAP-25 expression in cultured rat hippocampal explants, but not in cultured rat cerebellar explants | en |
Document type | dc.type | Artículo de revista |
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