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Authordc.contributor.authorEltit Ortega, José Miguel 
Authordc.contributor.authorGarcía, Alejandra es_CL
Authordc.contributor.authorHidalgo Tapia, Jorge es_CL
Authordc.contributor.authorLiberona Leppe, José es_CL
Authordc.contributor.authorChiong Lay, Mario es_CL
Authordc.contributor.authorLavandero González, Sergioes_CL
Authordc.contributor.authorMaldonado Maldonado, Edio es_CL
Authordc.contributor.authorJaimovich Pérez, Enrique 
Admission datedc.date.accessioned2009-06-23T10:55:11Z
Available datedc.date.available2009-06-23T10:55:11Z
Publication datedc.date.issued2006-04-28
Cita de ítemdc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY 281(17):12143-12154en
Identifierdc.identifier.issn0021-9258
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/120701
Abstractdc.description.abstractTetanic electrical stimulation of myotubes evokes a ryanodine receptor-related fast calcium signal, during the stimulation, followed by a phospholipase C/inositol 1,4,5-trisphosphate-dependent slow calcium signal few seconds after stimulus end. L-type calcium channels (Cav 1.1, dihydropyridine receptors) acting as voltage sensors activate an unknown signaling pathway involved in phospholipase C activation. We demonstrated that both G protein and phosphatidylinositol 3- kinase were activated by electrical stimulation, and both the inositol 1,4,5-trisphosphate rise and slow calcium signal induced by electrical stimulation were blocked by pertussis toxin, by a G beta gamma scavenger peptide, and by phosphatidylinositol 3- kinase inhibitors. Immunofluorescence using anti-phosphatidylinositol 3- kinase gamma antibodies showed a clear location in striations within the cytoplasm, consistent with a position near the I band region of the sarcomere. The time course of phosphatidylinositol 3- kinase activation, monitored in single living cells using a pleckstrin homology domain fused to green fluorescent protein, was compatible with sequential phospholipase C gamma 1 activation as confirmed by phosphorylation assays for the enzyme. Co-transfection of a dominant negative form of phosphatidylinositol 3- kinase gamma inhibited the phosphatidylinositol 3- kinase activity as well as the slow calcium signal. We conclude that G beta gamma/phosphatidylinositol 3- kinase gamma signaling pathway is involved in phospholipase C activation and the generation of the slow calcium signal induced by tetanic stimulation. We postulate that membrane potential fluctuations in skeletal muscle cells can activate a pertussis toxin-sensitive G protein, phosphatidylinositol 3- kinase, phospholipase C pathway toward modulation of long term, activity-dependent plastic changes.en
Lenguagedc.language.isoenen
Publisherdc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCen
Keywordsdc.subjectG-BETA-GAMMAen
Títulodc.titleMembrane electrical activity elicits inositol 1,4,5-trisphosphate-dependent slow Ca2+ signals through a G beta gamma/phosphatidylinositol 3-kinase gamma pathway in skeletal myotubesen
Document typedc.typeArtículo de revistaen


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