Author | dc.contributor.author | Letelier Muñoz, María Eugenia | |
Author | dc.contributor.author | Molina Berríos, Alfredo Enrique | es_CL |
Author | dc.contributor.author | Cortés Troncoso, Juan | es_CL |
Author | dc.contributor.author | Jara Sandoval, José | es_CL |
Author | dc.contributor.author | Holst, Marianne | es_CL |
Author | dc.contributor.author | Palma, Karina | es_CL |
Author | dc.contributor.author | Montoya, Margarita | es_CL |
Author | dc.contributor.author | Miranda, Dante | es_CL |
Author | dc.contributor.author | González Lira, Víctor | es_CL |
Admission date | dc.date.accessioned | 2010-03-09T14:11:59Z | |
Available date | dc.date.available | 2010-03-09T14:11:59Z | |
Publication date | dc.date.issued | 2008-03 | |
Cita de ítem | dc.identifier.citation | TOXICOLOGY IN VITRO, Volume: 22, Issue: 2, Pages: 279-286, 2008 | en_US |
Identifier | dc.identifier.issn | 0887-2333 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/120878 | |
Abstract | dc.description.abstract | Numerous investigations exist about the alterations that oxygen free radicals can provoke on biomolecules; these modifications can be
prevented and/or reversed by different antioxidants agents. On the other hand, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), a stable
nitrogen synthetic radical, is used to evaluate the antioxidant capacity of medicinal herbal products; however, the structural changes that
this radical provoke on the herbal active principles are not clear yet. In this work, we compared the redox reactivity of oxygen free radicals
and DPPH radical on phospholipids and protein thiol groups present in rat liver microsomes. Cu2+/ascorbate was used as generator
system of oxygen free radical and as antioxidant, an extract of Buddleja globosa’s leaves. Cu2+/ascorbate provoked microsomal lipid
peroxidation, microsomal thiols oxidation and oxygen consumption; all of these phenomena were inhibited by B. globosa extract. On
the other hand, DPPH was bleached in different extension by the herbal extract and phosphatidyl choline; beside, DPPH decreased
microsomal thiols content, but this phenomenon were not prevented by the herbal extract. Furthermore, DPPH did not induce oxygen
consumption and neither modified the oxygen consumption induced by Cu2+/ascorbate. Distinct redox mechanisms may explain the differences
between the reactivity of DPPH and oxygen free radicals on biomolecules, which is discussed. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | en_US |
Keywords | dc.subject | DPPH and oxygen free radicals | en_US |
Título | dc.title | DPPH and oxygen free radicals as pro-oxidant of biomolecules | en_US |
Document type | dc.type | Artículo de revista | |