Multiple calcium pathways induce the expression of SNAP-25 protein in chromaffin cells
Author | dc.contributor.author | García Palomero, E. | |
Author | dc.contributor.author | Montiel, C. | es_CL |
Author | dc.contributor.author | Herrero, C. J. | es_CL |
Author | dc.contributor.author | García, A. G. | es_CL |
Author | dc.contributor.author | Alvarez, R. M. | es_CL |
Author | dc.contributor.author | Arnalich, F. M. | es_CL |
Author | dc.contributor.author | Renart, J. | es_CL |
Author | dc.contributor.author | Lara Peñaloza, Hernán | es_CL |
Author | dc.contributor.author | Cárdenas, A. M. | es_CL |
Admission date | dc.date.accessioned | 2011-04-07T18:38:39Z | |
Available date | dc.date.available | 2011-04-07T18:38:39Z | |
Publication date | dc.date.issued | 2000-03 | |
Cita de ítem | dc.identifier.citation | JOURNAL OF NEUROCHEMISTRY 74 (3): 1049-1058 | en_US |
Identifier | dc.identifier.issn | 0022-3042 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/121166 | |
Abstract | dc.description.abstract | Incubation of bovine adrenal chromaffin cells in high K+ (38 mM) during 24-48 h enhanced 2.5 to five times the expression of SNAP-25 protein and mRNA, respectively. This increase was reduced 86% by furnidipine 9(an L-type Ca2+ channel blocker) but was unaffected by either w-conotoxin GVIA (an N-type Ca2+ channel blocker) or w-agatoxin IVA (a P/Q-type Ca2+ channel blocker). Combined blockade of N and P/Q channels with w-conotoxin MVIIC did, however, block by 76% the protein expression. The inhibitory effects of furnidipine were partially reversed when the external Ca2+ concentration was raised from 1.6 to 5 mM. These findings, together with the fact that nicotinic receptor activation or Ca2+ release from internal stores also enhanced SNAP-25 protein expression, suggest that an increment of cytosolic Ca2+ concentration ([Ca2+],), rather than its source or Ca2+ entry pathway, is the critical signal to induce the protein expression, The greater coupling between L-type Ca2+ channels and protein expression might be due to two facts: (a) L channels contributed 50% to the global [Ca2+], rise induced by 38 mM K+ in indo-1-loaded chromaffin cells and (b) L channels undergo less inactivation than N or P/Q channels on sustained stimulation of these cells. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | en_US |
Keywords | dc.subject | TYROSINE-HYDROXYLASE GENE | en_US |
Título | dc.title | Multiple calcium pathways induce the expression of SNAP-25 protein in chromaffin cells | en_US |
Document type | dc.type | Artículo de revista |
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