Gelatinase activitiy of matrix metalloproteinases in the cerebrospinal fluid of various patient populations
Author | dc.contributor.author | Valenzuela Pedevila, María Antonieta | |
Author | dc.contributor.author | Cartier Rovirosa, Luis | es_CL |
Author | dc.contributor.author | Collados, L. E. | es_CL |
Author | dc.contributor.author | Kettlun, Ana María | es_CL |
Author | dc.contributor.author | Araya, F. | es_CL |
Author | dc.contributor.author | Concha, C. | es_CL |
Author | dc.contributor.author | Flores, L. | es_CL |
Author | dc.contributor.author | Wolf, M. E. | es_CL |
Author | dc.contributor.author | Mosnaim, A. D. | es_CL |
Admission date | dc.date.accessioned | 2011-07-18T18:39:17Z | |
Available date | dc.date.available | 2011-07-18T18:39:17Z | |
Publication date | dc.date.issued | 1999 | |
Cita de ítem | dc.identifier.citation | RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY 104 (1): 42-52 | es_CL |
Identifier | dc.identifier.issn | 1078-0297 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/121401 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | We have studied the enzymatic gelatinolytic activity of matrix metalloproteinases (MMPs) present in cerebrospinal fluid (CSF) of samples obtained from 67 individuals, twenty-one nonneurological patients (considered controls) and 46 subjects with various neurological disorders e.g., vascular lesions, demyelination, inflammatory, degenerative and prion diseases. Biochemical characterization of MMPs, a family of neutral proteolytic enzymes involved in extracellular matrix modeling, included determination of substrate specificity and Ca+2 dependency, as well as the effects of protease inactivators, carboxylic and His (histidine) residue modifiers, and antibiotics. Whereas all CSF samples expressed MMP-2 (gelatinase A) activity, it corresponded in most cases (normal and pathological samples) to its latent form (proenzyme; pMMP-2). In general, inflammatory neurological diseases (especially meningitis and neurocisticercosis) were associated with the presence of a second enzyme, MMP-9 (or gelatinase B). Whereas MMP-9 was found in the CSF of every tropical spastic paraparesis patient studied, its presence in samples from individuals with vascular lesions was uncommon. Patients blood-brain barrier damage was ascertained by determining total CSF protein content using both, the conventional polyacrylamide gel electrophoresis procedure under denaturing conditions and capillary zone electrophoresis. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | P J D PUBLICATIONS LTD | es_CL |
Keywords | dc.subject | CENTRAL-NERVOUS-SYSTEM | es_CL |
Título | dc.title | Gelatinase activitiy of matrix metalloproteinases in the cerebrospinal fluid of various patient populations | es_CL |
Document type | dc.type | Artículo de revista |
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