A high-performance liquid chromatography method for determination 2-(n-(N,N,N-trimethyl)-n-alkyl)-5-alkylfuryl halides in dipalmitoylphosphatidilcholine liposome solutions
Author
dc.contributor.author
Morales, Javier
Author
dc.contributor.author
Zanocco Loyola, Antonio
es_CL
Author
dc.contributor.author
Günther Sapunar, Germán
es_CL
Author
dc.contributor.author
Lemp Miranda, Else
es_CL
Admission date
dc.date.accessioned
2014-01-07T19:23:03Z
Available date
dc.date.available
2014-01-07T19:23:03Z
Publication date
dc.date.issued
2006
Cita de ítem
dc.identifier.citation
J. Chromatogr. A 1125 (2006) 89–94
en_US
Identifier
dc.identifier.other
doi:10.1016/j.chroma.2006.05.031
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/121708
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
A high-performance liquid chromatography (HPLC) method for the determination of 2-(4-(N,N,N-trimethyl)-butyl)-5-dodecylfuryl bromide
(DFTA) in dipalmitoylphophatidil-choline (DPPC) liposome solutions has been developed. Lipid-soluble furan derivatives, 2,5-disubstituted with
different n-alkyl chains and a terminal trimethylammonium group are useful probes for studying singlet oxygen dynamics and equilibria in
microcompartmentalized systems. The actual HPLC method uses a gradient elution and DAD detection. The chromatographic separation of these
components is achieved using a C18 analytical column with a 10mM solution of 1-heptanesulfonic acid (PIC-7)–methanol (10:90, v/v) as initial
mobile phase. Both DFTA peaks are well resolved and free of interference from matrix components and reaction products. The method has been
found to be linear (r > 0.999) over a wide concentration range and reliable to perform kinetic experiments in which the time dependent consumption
of a tetraalkylammonium surfactant in a microorganized systems composed by lipidic surfactants is followed.
A high-performance liquid chromatography method for determination 2-(n-(N,N,N-trimethyl)-n-alkyl)-5-alkylfuryl halides in dipalmitoylphosphatidilcholine liposome solutions