Sensing immobilized molecules of streptavidin on a silicon surface by maldi-tof mass spectrometry and fluorescence microscopy
Author
dc.contributor.author
Araya, Eyleen
Author
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Kogan Bocian, Marcelo
es_CL
Author
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Guell, Aleix G.
es_CL
Author
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Escobar, Carlos A.
es_CL
Author
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Sanz, Fausto
es_CL
Admission date
dc.date.accessioned
2014-12-18T19:01:01Z
Available date
dc.date.available
2014-12-18T19:01:01Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
J. Chil. Chem. Soc., 59, Nº 2 (2014)
en_US
Identifier
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https://repositorio.uchile.cl/handle/2250/121923
General note
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Artículo de publicación ScIELO
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Abstract
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A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-
undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and
subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization
of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are
used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the
development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated
that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces
en_US
Patrocinador
dc.description.sponsorship
Financial support provided by Fondecyt
11130494, Fondap 15130011, DI-569-14/R, the Ministerio de Ciencia y
Tecnología (Spain) throughout the project DPI-2000-703-C03.