Author | dc.contributor.author | Hernández Ríos, Emma | |
Author | dc.contributor.author | Sorsa, Timo | es_CL |
Author | dc.contributor.author | Obregón, Fabián | es_CL |
Author | dc.contributor.author | Tervahartiala, Taina | es_CL |
Author | dc.contributor.author | Valenzuela, María Antonieta | es_CL |
Author | dc.contributor.author | Pozo, Patricia | es_CL |
Author | dc.contributor.author | Dutzan Muñoz, Nicolás | es_CL |
Author | dc.contributor.author | Lesaffre, Emmanuel | es_CL |
Author | dc.contributor.author | Molas, Marek | es_CL |
Author | dc.contributor.author | Gamonal Aravena, Jorge Antonio | es_CL |
Admission date | dc.date.accessioned | 2011-11-10T15:40:05Z | |
Available date | dc.date.available | 2011-11-10T15:40:05Z | |
Publication date | dc.date.issued | 2009-12 | |
Cita de ítem | dc.identifier.citation | J Clin Periodontol. 2009 Dec;36(12):1011-7 | es_CL |
Identifier | dc.identifier.issn | 0303-6979 | |
Identifier | dc.identifier.other | DOI: 10.1111/j.1600-051X.2009.01488.x | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/123413 | |
Abstract | dc.description.abstract | Aim: Matrix metalloproteinases (MMP)-13 can initiate bone resorption and activate proMMP-9 in vitro, and both these MMPs have been widely implicated in tissue destruction associated with chronic periodontitis. We studied whether MMP-13 activity and TIMP-1 levels in gingival crevicular fluid (GCF) associated with progression of chronic periodontitis assessed clinically and by measuring carboxy-terminal telopeptide of collagen I (ICTP) levels. We additionally addressed whether MMP-13 could potentiate gelatinase activation in diseased gingival tissue.
Materials and Methods: In this prospective study, GCF samples from subjects undergoing clinical progression of chronic periodontitis and healthy controls were screened for ICTP levels, MMP-13 activity and TIMP-1. Diseased gingival explants were cultured, treated or not with MMP-13 with or without adding CL-82198, a synthetic MMP-13 selective inhibitor, and assayed by gelatin zymography and densitometric analysis.
Results: Active sites demonstrated increased ICTP levels and MMP-13 activity (p<0.05) in progression subjects. The MMP-9 activation rate was elevated in MMP-13-treated explants (p<0.05) and MMP-13 inhibitor prevented MMP-9 activation.
Conclusions: MMP-13 could be implicated in the degradation of soft and hard supporting tissues and proMMP-9 activation during progression of chronic periodontitis. MMP-13 and -9 can potentially form an activation cascade overcoming the protective TIMP-1 shield, which may become useful for diagnostic aims and a target for drug development. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | European Federation of Periodontology | es_CL |
Keywords | dc.subject | chronic periodontitis progression | es_CL |
Título | dc.title | Proteolytic roles of matrix metalloproteinase (MMP)-13 during progression of chronic periodontitis: initial evidence for MMP-13/MMP-9 activation cascade | es_CL |
Document type | dc.type | Artículo de revista | |