Author | dc.contributor.author | Hernández Ríos, Emma | |
Author | dc.contributor.author | Valenzuela, María Antonieta | es_CL |
Author | dc.contributor.author | López Otin, Carlos | es_CL |
Author | dc.contributor.author | Alvarez, Jesús | es_CL |
Author | dc.contributor.author | López, José Manuel | es_CL |
Author | dc.contributor.author | Vernal Astudillo, Rolando | es_CL |
Author | dc.contributor.author | Gamonal Aravena, Jorge Antonio | es_CL |
Admission date | dc.date.accessioned | 2012-01-02T17:54:23Z | |
Available date | dc.date.available | 2012-01-02T17:54:23Z | |
Publication date | dc.date.issued | 2006-11 | |
Cita de ítem | dc.identifier.citation | J Periodontol. 2006 Nov;77(11):1863-70. | es_CL |
Identifier | dc.identifier.issn | 0303-6979 | |
Identifier | dc.identifier.other | doi:10.1902/jop.2006.050461 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/123428 | |
Abstract | dc.description.abstract | Background: Matrix metalloproteinases (MMPs) participate in extracellular matrix degradation in physiological and pathological conditions. The available evidence suggests that MMP-13 plays a significant role in both the initiation and progress of bone resorption. The aim of our study was to identify the presence of MMP-13 in adult patients with untreated chronic periodontitis. We also determined the activity of MMP-13 present in lesions undergoing episodic attachment loss in gingival crevicular fluid (GCF) samples. Methods: After monitoring at 2 and 4 months, 21 patients showed destructive periodontitis (periodontally affected sites presenting at least two sites with ≥2 mm clinical attachment loss), and GCF samples were collected both from active and inactive sites (21 GCF samples, each). GCF was collected during a 30-second interval using a paper strip, and an immunofluorescence assay was performed to determine the basal activity of MMP-13 and the relationship between 4-aminophenylmercuric acetate (APMA)-activated total MMP-13 and basal MMP-13 activity. Gingival tissues from five patients were fixed in formalin and MMP-13 expression was demonstrated using immunohistochemistry and in situ hybridization. MMP-13 molecular forms were examined by Western immunoblotting with monoclonal antibodies. Results: MMP-13 was found in 100% of GCF samples from patients with chronic periodontitis. Active sites, associated with tissue destruction, had significantly higher proportions of active MMP-13 and MMP-13 activity levels than their inactive counterparts (1.49 versus 1.17 ng fluorescent product, respectively; P <0.05). Western blot, immunohistochemical staining, and in situ hybridization confirmed the presence of MMP-13 in periodontal disease, with observable differences between periodontitis and healthy subjects. MMP-13 immunoreactivities were seen mainly as 55 and 48 kDa, corresponding to partially and fully activated forms, respectively, and a smaller proportion of 60-kDa proenzyme form. Conclusion: MMP-13 activity in GCF samples was significantly increased in active sites from progressive periodontal disease, supporting its role in the alveolar bone loss developed in this disease. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | American Academy of Periodontology | es_CL |
Keywords | dc.subject | Collagenase-3 | es_CL |
Título | dc.title | Matrix metalloproteinase-13 is highly expressed in destructive periodontal disease activity | es_CL |
Document type | dc.type | Artículo de revista | |