Neisseria gonorrhoeae Induces a Tolerogenic Phenotype in Macrophages to Modulate Host Immunity
Author
dc.contributor.author
Escobar Álvarez, Alejandro
es_CL
Author
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Candia, Enzo
es_CL
Author
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Reyes Cerpa, Sebastián
es_CL
Author
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Villegas Valdés, Bélgica
es_CL
Author
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Neira, Tanya
es_CL
Author
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López Nitsche, Mercedes
es_CL
Author
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Maisey, Kevin
es_CL
Author
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Tempio, Fabián
es_CL
Author
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Ríos, Miguel
es_CL
Author
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Acuña Castillo, Claudio
es_CL
Author
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Imarai, Mónica
Admission date
dc.date.accessioned
2014-02-14T17:11:59Z
Available date
dc.date.available
2014-02-14T17:11:59Z
Publication date
dc.date.issued
2013
Cita de ítem
dc.identifier.citation
Mediators of Inflammation Volume 2013, Article ID 127017
en_US
Identifier
dc.identifier.issn
doi 10.1155/2013/127017
Identifier
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https://repositorio.uchile.cl/handle/2250/123526
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
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Neisseria gonorrhoeae is the etiological agent of gonorrhoea, which is a sexually transmitted disease widespread throughout the
world. N. gonorrhoeae does not improve immune response in patients with reinfection, suggesting that gonococcus displays several
mechanisms to evade immune response and survive in the host. N. gonorrhoeae is able to suppress the protective immune response
at different levels, such as B and T lymphocytes and dendritic cells. In this study, we determined whether N. gonorrhoeae directly
conditions the phenotype of RAW 264.7 murine macrophage cell line and its response. We established that gonococcus was
effectively phagocytosed by the RAW 264.7 cells and upregulates production of immunoregulatory cytokines (IL-10 and TGF-𝛽1)
but not the production of proinflammatory cytokine TNF-𝛼, indicating that gonococcus induces a shift towards anti-inflammatory
cytokine production. Moreover, N. gonorrhoeae did not induce significant upregulation of costimulatory CD86 and MHC class II
molecules.We also showed that N. gonorrhoeae infectedmacrophage cell line fails to elicit proliferative CD4+ response.This implies
that macrophage that can phagocytose gonococcus do not display proper antigen-presenting functions. These results indicate that
N. gonorrhoeae induces a tolerogenic phenotype in antigen-presenting cells, which seems to be one of the mechanisms to induce
evasion of immune response.