Author | dc.contributor.author | Cornejo, Pamela | |
Author | dc.contributor.author | Varela, Patricia | es_CL |
Author | dc.contributor.author | Videla Cabrera, Luis | es_CL |
Author | dc.contributor.author | Fernández Arancibia, Virginia | es_CL |
Admission date | dc.date.accessioned | 2007-05-17T19:40:29Z | |
Available date | dc.date.available | 2007-05-17T19:40:29Z | |
Publication date | dc.date.issued | 2005-08 | |
Cita de ítem | dc.identifier.citation | NITRIC OXIDE-BIOLOGY AND CHEMISTRY 13 (1): 54-61 AUG 2005 | en |
Identifier | dc.identifier.issn | 1089-8603 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/127165 | |
Abstract | dc.description.abstract | Iron is an essential micronutrient promoting oxidative stress in the liver of overloaded animals and human, which may trigger the expression of redox-sensitive genes. We have tested the hypothesis that chronic iron overload (CIO) enhances inducible nitric oxide synthase (iNOS) expression in rat liver by extracellular signal-regulated kinase (ERK 1/2) and NF-kappa B activation. CIO (diet enriched with 3%(wt/wt) carbonyl-iron for 12 weeks) increased liver protein carbonylation and decreased reduced glutathione (GSH) content and the GSH/GSSG ratio after 6 weeks, parameters that are normalized after 8-12 weeks of treatment. These changes are paralleled by higher phosphorylated-ERK1/2 to non-phosphorylated-ERK1/2 ratios at 6 and 8 weeks, increased NF-kappa B DNA binding to the iNOS gene promoter at 8-12 weeks, and higher iNOS mRNA expression and activity at 8 and 12 weeks. It is concluded that CIO triggers liver oxidative stress at early times, with upregulation of iNOS expression involving the ERK/NF-kappa B pathway at later times, a finding that may represent a hepatoprotective mechanism against CIO toxicity in addition to the recovery of GSH horneostasis. | en |
Lenguage | dc.language.iso | en | en |
Publisher | dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en |
Keywords | dc.subject | PROXIMAL TUBULE CELLS | en |
Título | dc.title | Chronic iron overload enhances inducible nitric oxide synthase expression in rat liver | en |
Document type | dc.type | Artículo de revista | |