Author | dc.contributor.author | Ramírez Villalobos, Eugenio | es_CL |
Author | dc.contributor.author | Cartier Rovirosa, Luis | es_CL |
Author | dc.contributor.author | Torres, Mauricio | es_CL |
Author | dc.contributor.author | Barría, Marcelo | es_CL |
Admission date | dc.date.accessioned | 2008-05-14T14:07:10Z | |
Available date | dc.date.available | 2008-05-14T14:07:10Z | |
Publication date | dc.date.issued | 2007 | es_CL |
Cita de ítem | dc.identifier.citation | JOURNAL OF MEDICAL VIROLOGY Vol. 79 JUN 2007 6 782-790 | es_CL |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/127503 | |
General note | dc.description | Publicación ISI | es_CL |
Abstract | dc.description.abstract | Human T-cell lymphotropic virus type I (HTLV-1) is the etiologic agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). High HTLV-1 provirus load and tax mRNA level have been suggested as predictors of disease progression in patients with HAM/TSP, but little is known about the temporal variation in patients. To clarify the role of high proviral and tax mRNA loads and their fluctuations in the pathogenesis of HAM/TSP, we measured proviral load and tax mRNA in serially collected peripheral blood mononuclear cells (PBMCs) from nine patients with HAM/TSP during a long-term follow-up, by use of real-time polymerase chain reaction using tax primers. The real-time PCR quantitation revealed a wide range of variation of proviral loads (7.82-97.13 copies per 100 PBMCs) and tax mRNA (0.20-245.30 copies) among HAM/TSP patients. Patients showed three different patterns of HTLV-1 tax mRNA loads during the course of the disease. Tax mRNA load showed a separate evolution with respect to the disease. The dynamic patterns of proviral load and mRNA Tax expression suggest that only the permanent presence of a basal level of tax mRNA, rather than the tax mRNA load, is related to the development of HAM/TSP. To our knowledge, this is the first longitudinal study to determine tax mRNA expression at different clinical stages. J. Med. Viral. 79:782- 790, 2007. (C) 2007 Wiley-Liss, Inc. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Keywords | dc.subject | HTLV-I-associated myelopathy/tropical spastic paraparesis | es_CL |
Area Temática | dc.subject.other | Virology | es_CL |
Título | dc.title | Temporal dynamics of human T-lymphotropic virus type I tax mRNA and proviral DNA load in peripheral blood mononuclear cells of human T-lymphotropic virus type I-Associated myelopathy patients | es_CL |
Document type | dc.type | Artículo de revista | |