Author | dc.contributor.author | Sandoval, Mauricio | es_CL |
Author | dc.contributor.author | Sandoval, Rodrigo | es_CL |
Author | dc.contributor.author | Thomas, Ulrich | es_CL |
Author | dc.contributor.author | Spilker, Christina | es_CL |
Author | dc.contributor.author | Smalla, Karl-Heinz | es_CL |
Author | dc.contributor.author | Falcón, Romina | es_CL |
Author | dc.contributor.author | Marengo, Juan José | es_CL |
Author | dc.contributor.author | Calderón, Rodrigo | es_CL |
Author | dc.contributor.author | Saavedra, Verónica | es_CL |
Author | dc.contributor.author | Heumann, Rolf | es_CL |
Author | dc.contributor.author | Bronfman, Francisca | es_CL |
Author | dc.contributor.author | Garner, Craig C. | es_CL |
Author | dc.contributor.author | Gundelfinger, Eckart D. | es_CL |
Author | dc.contributor.author | Wyneken, Ursula | es_CL |
Admission date | dc.date.accessioned | 2008-05-14T14:11:11Z | |
Available date | dc.date.available | 2008-05-14T14:11:11Z | |
Publication date | dc.date.issued | 2007 | es_CL |
Cita de ítem | dc.identifier.citation | JOURNAL OF NEUROCHEMISTRY Vol. 101 JUN 2007 6 1672-1684 | es_CL |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/127527 | |
General note | dc.description | Publicación ISI | es_CL |
Abstract | dc.description.abstract | Brain-derived neurotrophic factor (BDNF) and its receptor TrkB are essential regulators of synaptic function in the adult CNS. A TrkB-mediated effect at excitatory synapses is enhancement of NMDA receptor (NMDA-R)-mediated currents. Recently, opposing effects of TrkB and the pan-neurotrophin receptor p75(NTR) on long-term synaptic depression and long-term potentiation have been reported in the hippocampus. To further study the regulation of NMDA-Rs by neurotrophin receptors in their native protein environment, we micro-transplanted rat forebrain post-synaptic densities (PSDs) into Xenopus oocytes. One-minute incubations of oocytes with BDNF led to dual effects on NMDA-R currents: either TrkB-dependent potentiation or TrkB-independent inhibition were observed. Pro-nerve growth factor, a ligand for p75(NTR) but not for TrkB, produced a reversible, dose-dependent, TrkB-independent and p75(NTR)-dependent inhibition of NMDA-Rs. Fractionation experiments showed that p75(NTR) is highly enriched in the PSD protein fraction. Immunoprecipitation and pull-down experiments further revealed that p75(NTR) is a core component of the PSD, where it interacts with the PDZ3 domain of the scaffolding protein SAP90/PSD-95. Our data provide striking evidence for a rapid inhibitory effect of p75(NTR) on NMDA-R currents that antagonizes TrkB-mediated NMDA-R potentiation. These opposing mechanisms might be present in a large proportion of forebrain synapses and may contribute importantly to synaptic plasticity. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Keywords | dc.subject | brain-derived neurotrophic factor | es_CL |
Area Temática | dc.subject.other | Biochemistry & Molecular Biology; Neurosciences | es_CL |
Título | dc.title | Antagonistic effects of TrkB and p75(NTR) on NMDA receptor currents in post-synaptic densities transplanted into Xenopus oocytes | es_CL |
Document type | dc.type | Artículo de revista | |