Author | dc.contributor.author | Horta, Fabrizzio | |
Author | dc.contributor.author | Madariaga, Marcia | es_CL |
Author | dc.contributor.author | García, Alejandra | es_CL |
Author | dc.contributor.author | Härtel, Steffen | es_CL |
Author | dc.contributor.author | Smith Garcés, Rosita | es_CL |
Admission date | dc.date.accessioned | 2011-11-14T19:45:15Z | |
Available date | dc.date.available | 2011-11-14T19:45:15Z | |
Publication date | dc.date.issued | 2011-03 | |
Cita de ítem | dc.identifier.citation | REVISTA MEDICA DE CHILE Volume: 139 Issue: 3 Pages: 306-312 Published: MAR 2011 | es_CL |
Identifier | dc.identifier.issn | 0034-9887 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128894 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | Background: There is an association between aging and an increased number of sperms with alterations in nuclear DNA. Aim: To study the association between age and fragmentation of sperm DNA. Material and Methods: Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersion test) assays. Results: Compared with their younger counterparts, patients aged more than 40 years had a higher proportion of sperms with DNA fragmentation by TUNEL (20 +/- 1.3 and 24 +/- 1.9% respectively, p < 0.05) and SCD (22 +/- 1.4 and 26 +/- 1.6% respectively, p < 0.05). The results of both assays had a correlation coefficient of 0.8. No differences between groups were observed for other seminal parameters. Conclusions: Sperm DNA fragmentation increases with age in males. | es_CL |
Patrocinador | dc.description.sponsorship | FONDEF
D07I1019/FONDECYT
1070756. | es_CL |
Lenguage | dc.language.iso | es | es_CL |
Publisher | dc.publisher | SOC MEDICA SANTIAGO | es_CL |
Keywords | dc.subject | Aging | es_CL |
Título | dc.title | Aumento del daño en el ADN espermático en varones mayores de 40 años | es_CL |
Title in another language | dc.title.alternative | Association of age with sperm DNA fragmentation | es_CL |
Document type | dc.type | Artículo de revista | |