Estudio in vivo del efecto angiogénico de inhibidor de fosfodiesterasa para aplicación posterior en injerto dermoepidérmico

Abstract

Background: Angiogenesis is a complex process. Phosphodiesterase inhibitors may have a direct angiogenic effect. Aim: To determine if phosphodiesterase inhibitors have angiogenic properties, using a chicken egg model. Material and Methods: We used 44 fertilized chicken eggs. A methylcellulose filter was placed over their allantocorionic membrane. This preparation was instilled with different solutions. Group A (Control) received 30 mu l of saline solution, Group B, C and D received 30 mu l of a solution made of saline solution and sildenafil at different concentrations of 0.33, 1 and 3.3 mu g/mu l, respectively. At day 12 the filters were removed, prepared for histologic analysis, and the number of capillaries in an area of 2250 mu m(2) were blindly counted. Statistical analysis was made using variance analysis (ANOVA) with Bonferroni technique (p < 0.001). Results: The number of capillaries counted, per 2250 mu m(2), in Groups A, B, C, and D were 11.1 +/- 0.5, 15.4 +/- 1.2, 16.6 +/- 0.8 and 19.2 +/- 0.9, respectively. The number of capillaries of groups 13, C and D were significantly higher than those of group A (control). Moreover, there was a linear relationship between the number of capillaries and sildenafil dose (p < 0.001). Conclusions: In this experiment, sildenafil had a potent angiogenic effect.