Author | dc.contributor.author | Rodríguez Bustos, Héctor | es_CL |
Author | dc.contributor.author | Espinoza Navarro, Omar | es_CL |
Author | dc.contributor.author | Silva, Iván | es_CL |
Author | dc.contributor.author | Needham, Douglas | es_CL |
Author | dc.contributor.author | Castro, María Eugenia | es_CL |
Author | dc.contributor.author | Sarabia, Luis | es_CL |
Author | dc.contributor.author | Inostroza, Juan | |
Author | dc.contributor.author | Jiménez, Leonella | es_CL |
Admission date | dc.date.accessioned | 2012-01-02T20:40:50Z | |
Available date | dc.date.available | 2012-01-02T20:40:50Z | |
Publication date | dc.date.issued | 2011 | |
Cita de ítem | dc.identifier.citation | Environ Sci Pollut Res (2011) 18:497–502 | es_CL |
Identifier | dc.identifier.other | DOI 10.1007/s11356-010-0385-0 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128935 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | Introduction and aims The Chilean Altiplano ecosystem is
conserved free from contaminants and pollutants because of
the absence of major local human activities such as
agriculture or other industries. We studied the effects of
paraoxon on proliferation and apoptosis of testicular cells during active spermatogenesis in Dugesia gonocephala
collected from a pristine river (Guacollo) in the Altiplano
region nearby Visviri town, Chile.
Materials and methods Adult planarians were incubated in
varying concentrations of paraoxon (0.8, 0.4, 0.04, 0.004,
and 0.0004 mM) for 4 h. After 3 h of incubation,
bromodeoxyuridine (BrdU) was added. Effects on cell
proliferation (BrdU) and apoptosis (Apaf-1) were determined
by immunohistochemistry.
Results Paraoxon concentrations of 0.4 and 0.8 mM caused
100% mortality in the respective treatment groups. The lowest
tested concentration (0.0004 mM) caused a significant
increase on cell proliferation in the seminiferous tubules, as
well as an increase in the number of apoptotic cells. All other
tested concentrations significantly inhibited cell proliferation
and induced apoptosis.
Conclusions Paraoxon inhibits DNA synthesis and induces
apoptosis during spermatogenesis in adult planarians from a
high-altitude, pollution-free environment. This could
suggest its use as a biosensor or biomarker for contamination
with agro pesticides. | es_CL |
Patrocinador | dc.description.sponsorship | Universidad de Tarapacá, research project no. 4792 and FONDECYT
1101046- 2010. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | Springer | es_CL |
Keywords | dc.subject | High altitude | es_CL |
Título | dc.title | The effect of paraoxon on spermatogenesis in Dugesia gonocephala from the Chilean Altiplano: proliferation and apoptosis | es_CL |
Document type | dc.type | Artículo de revista | |