Author | dc.contributor.author | Riquelme, Denise | |
Author | dc.contributor.author | Álvarez, Alvaro | es_CL |
Author | dc.contributor.author | Leal, Nancy | es_CL |
Author | dc.contributor.author | Adasme, Tatiana | es_CL |
Author | dc.contributor.author | Espinoza, Italo | es_CL |
Author | dc.contributor.author | Valdés, Juan Antonio | es_CL |
Author | dc.contributor.author | Troncoso, Natalia | es_CL |
Author | dc.contributor.author | Härtel, Steffen | es_CL |
Author | dc.contributor.author | Hidalgo Tapia, Jorge | es_CL |
Author | dc.contributor.author | Hidalgo Tapia, María Cecilia | es_CL |
Author | dc.contributor.author | Carrasco Friz, María Angélica | es_CL |
Admission date | dc.date.accessioned | 2012-01-04T14:13:44Z | |
Available date | dc.date.available | 2012-01-04T14:13:44Z | |
Publication date | dc.date.issued | 2011 | |
Cita de ítem | dc.identifier.citation | ANTIOXIDANTS & REDOX SIGNALING Volume 14, Number 7, 2011 | es_CL |
Identifier | dc.identifier.other | DOI: 10.1089=ars.2010.3238 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128937 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | Neuronal electrical activity increases intracellular Ca2+ concentration and generates reactive oxygen species.
Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca2+ signals
with an early and a late component, and promoted hydrogen peroxide generation via a neuronal NADPH
oxidase. Hydrogen peroxide generation required both Ca2+ entry through N-methyl-D-aspartate receptors and
Ca2+ release mediated by ryanodine receptors (RyR). Field stimulation also enhanced nuclear translocation of the
NF-kB p65 protein and NF-kB -dependent transcription, and increased c-fos mRNA and type-2 RyR protein
content. Preincubation with inhibitory ryanodine or with the antioxidant N-acetyl L-cysteine abolished the
increase in hydrogen peroxide generation and the late Ca2+ signal component induced by electrical stimulation.
Primary cortical cells behaved similarly as primary hippocampal cells. Exogenous hydrogen peroxide also
activated NF-kB-dependent transcription in hippocampal neurons; inhibitory ryanodine prevented this effect.
Selective inhibition of the NADPH oxidase or N-acetyl L-cysteine also prevented the enhanced translocation of
p65 in hippocampal cells, while N-acetyl L-cysteine abolished the increase in RyR2 protein content induced by
high frequency stimulation. In conclusion, the present results show that electrical stimulation induced reciprocal
activation of ryanodine receptor-mediated Ca2+ signals and hydrogen peroxide generation, which stimulated
jointly NF-kB activity. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Título | dc.title | High-Frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-Mediated Ca2+ Release and Generates Hydrogen Peroxide, Which Jointly Stimulate NF-kB Activity | es_CL |
Document type | dc.type | Artículo de revista | |