| Author | dc.contributor.author | Husen, Peter | |
| Author | dc.contributor.author | Fidorra, Matthias | es_CL |
| Author | dc.contributor.author | Härtel, Steffen | es_CL |
| Author | dc.contributor.author | Bagatolli, Luis A. | es_CL |
| Author | dc.contributor.author | Ipsen, Jhon H. | es_CL |
| Admission date | dc.date.accessioned | 2012-06-05T15:58:46Z | |
| Available date | dc.date.available | 2012-06-05T15:58:46Z | |
| Publication date | dc.date.issued | 2011-11-09 | |
| Cita de ítem | dc.identifier.citation | Eur Biophys J (2012) 41:161–175 | es_CL |
| Identifier | dc.identifier.other | DOI 10.1007/s00249-011-0768-2 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128971 | |
| General note | dc.description | Artículo de publicación ISI | es_CL |
| Abstract | dc.description.abstract | Quantitative characterization of the lateral
structure of curved membranes based on fluorescence
microscopy requires knowledge of the fluorophore distribution
on the surface. We present an image analysis
approach for extraction of the fluorophore distribution on a
spherical lipid vesicle from confocal imaging stacks. The
technique involves projection of volumetric image data
onto a triangulated surface mesh representation of the
membrane, correction of photoselection effects and global
motion of the vesicle during image acquisition and segmentation
of the surface into domains using histograms.
The analysis allows for investigation of the morphology
and size distribution of domains on the surface. | es_CL |
| Patrocinador | dc.description.sponsorship | MEMPHYS Center for Biomembrane Physics
is supported by the Danish National Research Foundation. Research
in SCIAN-Lab (S.H.) is funded by FONDECYT (1090246) and
FONDEF (D07I1019), both CONICYT (Chile) and the Millennium
Scientific Initiative (ICM P09-015-F). The collaboration between the
L.A.B. and S.H. laboratories is supported by FONDECYT (1090246)
and MEMPHYS, Center for Biomembrane Physics, University of
Southern Denmark, Odense, Denmark. | es_CL |
| Lenguage | dc.language.iso | en_US | es_CL |
| Publisher | dc.publisher | Springer | es_CL |
| Keywords | dc.subject | Confocal microscopy | es_CL |
| Título | dc.title | A method for analysis of lipid vesicle domain structure from confocal image data | es_CL |
| Document type | dc.type | Artículo de revista | |
