| Author | dc.contributor.author | Lefimil, Claudia | |
| Author | dc.contributor.author | Jedlicki Corbeaux, Eugenia | es_CL |
| Author | dc.contributor.author | Holmes, David S. | es_CL |
| Admission date | dc.date.accessioned | 2012-06-13T21:24:12Z | |
| Available date | dc.date.available | 2012-06-13T21:24:12Z | |
| Publication date | dc.date.issued | 2012 | |
| Cita de ítem | dc.identifier.citation | Analytical Biochemistry 421 (2012) 333–335 | es_CL |
| Identifier | dc.identifier.other | doi:10.1016/j.ab.2011.10.019 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128974 | |
| General note | dc.description | Artículo de publicación ISI | es_CL |
| Abstract | dc.description.abstract | Reporter gene assays are important tools for evaluating gene expression. A frequently used assay measures
the activity of b-galactosidase (b-gal) expressed from lacZ in plasmid or genomic constructions.
Such constructions are often used to interrogate the ability of DNA (query DNA), potentially encoding
a transcription factor, to regulate in trans the expression of a promoter fused to the reporter lacZ. Query
DNA is frequently inserted into a second plasmid within the a-subunit of b-gal, interrupting its function.
However, this plasmid can induce up-expression of b-gal even when void of query DNA, leading to confusion
between artifact and authentic regulation. | es_CL |
| Patrocinador | dc.description.sponsorship | Fondecyt (1090451), Universidad Andres
Bello (DI-15-06-I), and Conicyt Basal (CCTE PFB16) | es_CL |
| Lenguage | dc.language.iso | en | es_CL |
| Publisher | dc.publisher | Elsevier | es_CL |
| Keywords | dc.subject | Gene reporter | es_CL |
| Título | dc.title | An artifact in studies of gene regulation using b-galactosidase reporter gene assays | es_CL |
| Document type | dc.type | Artículo de revista | |
