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Authordc.contributor.authorKong, Milene 
Authordc.contributor.authorMuñoz, Nicolás es_CL
Authordc.contributor.authorValdivia, Alejandra es_CL
Authordc.contributor.authorÁlvarez, Ávaro es_CL
Authordc.contributor.authorHerrera Molina, Rodrigo es_CL
Authordc.contributor.authorCárdenas, Areli es_CL
Authordc.contributor.authorSchneider, Pascal es_CL
Authordc.contributor.authorBurridge, Keith es_CL
Authordc.contributor.authorQuest, Andrew F. G. es_CL
Authordc.contributor.authorLeyton Campos, Lisette es_CL
Cita de ítemdc.identifier.citationBiochimica et Biophysica Acta 1833 (2013) 1409–1420en_US
Identifierdc.identifier.otherdoi: 10.1016/j.bbamcr.2013.02.013
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractCell adhesion to the extracellular matrix proteins occurs through interactions with integrins that bind to Arg-Gly-Asp (RGD) tripeptides, and syndecan-4, which recognizes the heparin-binding domain of other proteins. Both receptors trigger signaling pathways, including those that activate RhoGTPases such as RhoA and Rac1. This sequence of events modulates cell adhesion to the ECM and cell migration. Using a neuron– astrocyte model, we have reported that the neuronal protein Thy-1 engages αVβ3 integrin and syndecan-4 to induce RhoA activation and strong astrocyte adhesion to their underlying substrate. Thus, because cell–cell interactions and strong cell attachment to thematrix are considered antagonistic to cell migration, we hypothesized that Thy-1 stimulation of astrocytes should preclude cellmigration.Here, we studiedthe effect of Thy-1 expressing neurons on astrocyte polarization and migration using a wound-healing assay and immunofluorescence analysis. Signaling molecules involved were studied by affinity precipitation, western blotting and the usage of specific antibodies. Intriguingly, Thy-1 interaction with its two receptors was found to increase astrocyte polarization and migration. The latter events required interactions of these receptors with both the RGD-like sequence and the heparin-binding domain of Thy-1. Additionally, prolonged Thy-1-receptor interactions inhibited RhoA activation while activating FAK, PI3K and Rac1. Therefore, sustained engagement of integrin and syndecan-4with the neuronal surface protein Thy-1 induces astrocytemigration. Interestingly we identify here, a cell–cell interaction that despite initially inducing strong cell attachment, favors cellmigration upon persistent stimulation by engaging the same signaling receptors and molecules as those utilized by the extracellular matrix proteins to stimulate cell movement.en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleThy-1-mediated cell–cell contact induces astrocyte migration through the engagement of αVβ3 integrin and syndecan-4en_US
Document typedc.typeArtículo de revista

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile