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Authordc.contributor.authorCastillo, Christian 
Authordc.contributor.authorVillarroel, Arturo es_CL
Authordc.contributor.authorDuaso, Juan es_CL
Authordc.contributor.authorGalanti Garrone, Norbel es_CL
Authordc.contributor.authorCabrera Vallejos, Gonzalo es_CL
Authordc.contributor.authorMaya Arango, Juan es_CL
Authordc.contributor.authorKemmerling Weis, Ulrike es_CL
Admission datedc.date.accessioned2014-03-06T20:16:31Z
Available datedc.date.available2014-03-06T20:16:31Z
Publication datedc.date.issued2013
Cita de ítemdc.identifier.citationExperimental Parasitology 133 (2013) 12–17en_US
Identifierdc.identifier.otherdoi 10.1016/j.exppara.2012.10.012
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/129291
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractChagas‘ disease is caused by the haemophlagelated protozoan Trypanosoma cruzi (T. cruzi). During congenital transmission the parasite breaks down the placental barrier, however studies about the physiopathology of this process are scarce. Different signal transduction pathways are involved during cell invasion of the parasite. However, the possible role of those processes during tissue infection has not been studied. In the present study we analyzed the modulation of two signal transduction pathways, PLC-c and ERK1/2 MAPK, during ex vivo infection of human placental chorionic villi explants. Chorionic villi from healthy woman placentas were incubated in the presence or absence of 105 or 106 T. cruzi trypomastigotes (DM28c strain) with or without specific inhibitors for each pathway. Effective infection was tested determining parasite DNA by PCR. The activation of PLC-c and ERK1/2 MAPK signaling pathways was determined by western blotting and immunofluorescence. The low concentration of T. cruzi trypomastigotes activates both signaling pathways; however, the high concentration of parasite induces a modest activation of the PLC-c pathway and impairs the ERK1/2 MAPK pathway activation. Interestingly, inhibition of any of those signaling pathways did not prevent parasite infection, as it was previously shown in cell cultures. We conclude that both signal transduction pathways are modulated during ex vivo T. cruzi infection of human placental chorionic villi explants.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherElsevieren_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectTrypanosoma cruzien_US
Títulodc.titlePhospholipase C gamma and ERK1/2 Mitogen Activated Kinase Pathways are differentially modulated by Trypanosoma cruzi during tissue invasion in human placentaen_US
Document typedc.typeArtículo de revista


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile