The Expression of RAC1 and Mineralocorticoid Pathway- Dependent Genes are Associated With Different Responses to Salt Intake
Author | dc.contributor.author | Tapia Castillo, Alejandra | |
Author | dc.contributor.author | Carvajal, Cristian | |
Author | dc.contributor.author | Campino, Carmen | |
Author | dc.contributor.author | Hill, Caroline | |
Author | dc.contributor.author | Allende, Fidel | |
Author | dc.contributor.author | Vecchiola, Andrea | |
Author | dc.contributor.author | Carrasco, Carmen | |
Author | dc.contributor.author | Bancalari, Rodrigo | |
Author | dc.contributor.author | Valdivia, Carolina | |
Author | dc.contributor.author | Lagos, Carlos | |
Author | dc.contributor.author | Martínez Aguayo, Alejandro | |
Author | dc.contributor.author | García, Hernán | |
Author | dc.contributor.author | Aglony, Marlene | |
Author | dc.contributor.author | Baudrand, René | |
Author | dc.contributor.author | Kalergis, Alexis | |
Author | dc.contributor.author | Michea Acevedo, Luis | |
Author | dc.contributor.author | Riedel, Claudia | |
Author | dc.contributor.author | Fardella, Carlos | |
Admission date | dc.date.accessioned | 2015-08-05T19:23:37Z | |
Available date | dc.date.available | 2015-08-05T19:23:37Z | |
Publication date | dc.date.issued | 2015 | |
Cita de ítem | dc.identifier.citation | American Journal of Hypertension 28(6) June 2015 | en_US |
Identifier | dc.identifier.issn | 1941-7225 | |
Identifier | dc.identifier.other | DOI: 10.1093/ajh/hpu224 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/132448 | |
General note | dc.description | Artículo de publicación ISI | en_US |
Abstract | dc.description.abstract | BACKGROUND Rac1 upregulation has been implicated in salt-sensitive hypertension as a modulator of mineralocorticoid receptor (MR) activity. Rac1 could affect the expression of oxidative stress markers, such as hemoxigenase-1 (HO1) or nuclear factor-B (NF-kappa B), and the expression of neutrophil gelatinaseassociated lipocalin (NGAL), a cytokine upregulated upon MR activation. AIM We evaluated RAC1 expression in relation of high salt intake and association with MR, NGAL, HO-1, and NF-kappa B expression, mineralo-and glucocorticoids levels, and inflammatory parameters. SUBJECTS AND METHODS We studied 147 adult subjects. A food survey identified the dietary sodium (Na) intake. RAC1 expression was considered high or low according to the value found in normotensive subjects with low salt intake. We determined the gene expression of RAC1, MR, NGAL, HO-1, NF-kappa B, and 18S, isolated from peripheral leukocytes. We measured aldosterone, cortisol, sodium, potassium excretion, metalloproteinase (MMP9 y MMP2), and C-reactive protein. RESULTS We identified 126 subjects with high Na-intake, 18 subjects had high, and 108 low-RAC1 expression. The subjects with high-RAC1 expression showed a significant increase in MR (P = 0.0002), NGAL (P < 0.0001) HO-1 (P = 0.0004), and NF-kappa B (P < 0.0001) gene expression. We demonstrated an association between RAC1 expression and MR (R-sp 0.64; P < 0.0001), NGAL (R-sp 0.48; P < 0.0001), HO-1 (R-sp 0.53; P < 0.0001), and NF-kappa B (Rsp0.52; P < 0.0001). We did not identify any association between RAC1 and clinical or biochemical variables. CONCLUSIONS RAC1 expression was associated with an increase in MR, NGAL, NF-kappa B, and HO-1 expression, suggesting that RAC1 could be a mediator of cardiovascular damage induced by sodium, and may also useful to identify subjects with different responses to salt intake. | en_US |
Patrocinador | dc.description.sponsorship | Chilean grant FONDECYT 1130427 Chilean grant FONDEF-IDeA CA12i10150 Chilean grant SOCHED 2012-04 Chilean grant CORFO 13CTI-21526-P1 Millennium Institute of Immunology and Immunotherapy (MIII) P09/016-F | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | Oxford University Press | en_US |
Type of license | dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
Keywords | dc.subject | Blood pressure | en_US |
Keywords | dc.subject | Essential hypertension | en_US |
Keywords | dc.subject | Hypertension | en_US |
Keywords | dc.subject | PBMC | en_US |
Keywords | dc.subject | RAC1 | en_US |
Keywords | dc.subject | Gene expression | en_US |
Keywords | dc.subject | Salt intake | en_US |
Título | dc.title | The Expression of RAC1 and Mineralocorticoid Pathway- Dependent Genes are Associated With Different Responses to Salt Intake | en_US |
Document type | dc.type | Artículo de revista |
Files in this item
This item appears in the following Collection(s)
-
Artículos de revistas
Artículos de revistas