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Authordc.contributor.authorMaldonado Maldonado, Edio 
Authordc.contributor.authorRojas, Diego A. 
Authordc.contributor.authorMoreira Ramos, Sandra 
Authordc.contributor.authorUrbina, Fabiola 
Authordc.contributor.authorMiralles, Vicente J. 
Authordc.contributor.authorSolari Illescas, Aldo 
Authordc.contributor.authorVenegas Hermosilla, Juan Antonio 
Cita de ítemdc.identifier.citationParasitol Res (2015) 114:1313–1326en_US
Identifierdc.identifier.otherDOI: 10.1007/s00436-014-4308-8
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractChagas disease, caused by the protozoan Trypanosoma cruzi, is a major parasitic disease that affects millions of people in America. However, despite the high impact of this disease on human health, no effective and safe treatment has been found that eliminates the infecting parasite from human patients. Among the possible chemotherapeutic targets that could be considered for study in T. cruzi are the DNA polymerases, in particular DNA polymerase beta (polß), which previous studies have shown to be involved in kinetoplast DNA replication and repair. In this paper, we describe the expression, purification, and biochemical characterization of the Miranda clone polß, corresponding to lineage T. cruzi I (TcI). The recombinant enzyme purified to homogeneity displayed specific activity in the range described for a highly purified mammalian polß. However, the trypanosome enzyme exhibited important differences in biochemical properties compared to the mammalian enzymes, specifically an almost absolute dependency on KCl, high sensitivity to Nethylmaleimide (NEM), and low sensitivity to ddTTP. Immuno-affinity purification of T. cruzi polymerase beta (Tcpolß) from epimastigote extracts showed that the native enzyme was phosphorylated. In addition, it was demonstrated that Tcpolß interacts with some proteins in a group of about 15 proteins which are required to repair 1–6 bases of gaps of a double strand damaged DNA. It is possible that these proteins form part of a DNA repair complex, analogous to that described in mammals and some trypanosomatids.en_US
Patrocinadordc.description.sponsorshipFONDECYT, CONICYT-COLCIENCIAS Grant PCCICen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectDNA repairen_US
Keywordsdc.subjectRecombinant proteinen_US
Keywordsdc.subjectChagas diseaseen_US
Keywordsdc.subjectDNA polymeraseen_US
Keywordsdc.subjectTrypanosoma cruzien_US
Títulodc.titleExpression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native formen_US
Document typedc.typeArtículo de revista

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile