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Authordc.contributor.authorValenzuela Báez, Rodrigo 
Authordc.contributor.authorBarrera Ramírez, Cynthia 
Authordc.contributor.authorEspinosa, Alejandra 
Authordc.contributor.authorLlanos Vidal, Paola 
Authordc.contributor.authorOrellana, Paula 
Authordc.contributor.authorVidela Cabrera, Luis 
Cita de ítemdc.identifier.citationProstaglandins, Leukotrienes and Essential Fatty Acids 98 (2015) 7–14en_US
Identifierdc.identifier.otherDOI: 10.1016/j.plefa.2015.04.002
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractalpha-Linolenic (ALA) and linoleic (LA) acids are precursors of long chain polyunsaturated fatty acids (LCPUFAs), FAs with important biochemical and physiological functions. In this process, desaturation reactions catalyzed by Delta 5- and Delta 6-desaturase play a major role, enzymes that are subjected to hormonal and dietary regulation. The aim of this study was to assess the influence of a high fat diet (HFD) on activity of liver Delta 5 and Delta 6 desaturases, in relation to LCPUFA composition in liver and extrahepatic tissues. Male C57BL/6J mice received control diet (CD) (10% fat, 20% protein and 70% carbohydrate) or high fat diet (HFD) (60% fat, 20% protein, and 20% carbohydrate) for 12 weeks. After this time, blood and liver samples were taken for metabolic, morphologic, inflammatory, oxidative stress and desaturase activity assessment, besides FA phospholipid analysis in erythrocytes, heart, adipose tissue and brain. HFD significantly increased hepatic total fat, triacylglycerides and free FA content with macrovesicular steatosis and oxidative stress enhancement, concomitantly with higher fasting serum glucose and insulin levels, HOMA, and serum cholesterol, triacylglycerols, TNF-alpha, and IL-6. Diminution in liver Delta 5- and Delta 6-desaturase activities and LCPUFA depletion were induced by HFD, the later finding being also observed in extrahepatic tissues. In conclusion, HFD-induced reduction in the bioavailability of liver LCPUFA is associated with defective desaturation of ALA and LA, with Delta 5- and Delta 6-desaturase activities being correlated with insulin resistance development. Data analyzed point to the liver as a major organ responsible for extrahepatic LCPUFA homeostasis, which is markedly deranged by HFD.en_US
Patrocinadordc.description.sponsorshipChilean Endocrinology and Diabetes Society SOCHED 2013-04 PROYECTen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectLong-chain polyunsaturated fatty acidsen_US
Keywordsdc.subjectHigh-fat dieten_US
Keywordsdc.subjectLiver steatosisen_US
Keywordsdc.subjectDelta 5/Delta 6 desaturase activityen_US
Keywordsdc.subjectOxidative stressen_US
Keywordsdc.subjectExtrahepatic tissuesen_US
Títulodc.titleReduction in the desaturation capacity of the liver in mice subjected to high fat diet: Relation to LCPUFA depletion in liver and extrahepatic tissuesen_US
Document typedc.typeArtículo de revista

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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile