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Authordc.contributor.authorGonzález Roca, Carolina 
Authordc.contributor.authorCánovas, José 
Authordc.contributor.authorFresno, Javiera 
Authordc.contributor.authorCouve, Eduardo 
Authordc.contributor.authorCourt, Felipe A. 
Authordc.contributor.authorCouve Correa, Andrés 
Admission datedc.date.accessioned2016-06-14T13:49:54Z
Available datedc.date.available2016-06-14T13:49:54Z
Publication datedc.date.issued2016
Cita de ítemdc.identifier.citationPNAS Volumen: 113 Número: 7 Páginas: 1823-1828 (2016)en_US
Identifierdc.identifier.otherDOI: 10.1073/pnas.1514943113
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/138795
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractThe regulation of the axonal proteome is key to generate and maintain neural function. Fast and slow axoplasmic waves have been known for decades, but alternative mechanisms to control the abundance of axonal proteins based on local synthesis have also been identified. The presence of the endoplasmic reticulum has been documented in peripheral axons, but it is still unknown whether this localized organelle participates in the delivery of axonal membrane proteins. Voltage-gated sodium channels are responsible for action potentials and are mostly concentrated in the axon initial segment and nodes of Ranvier. Despite their fundamental role, little is known about the intracellular trafficking mechanisms that govern their availability in mature axons. Here we describe the secretory machinery in axons and its contribution to plasma membrane delivery of sodium channels. The distribution of axonal secretory components was evaluated in axons of the sciatic nerve and in spinal nerve axons after in vivo electroporation. Intracellular protein trafficking was pharmacologically blocked in vivo and in vitro. Axonal voltage-gated sodium channel mRNA and local trafficking were examined by RT-PCR and a retention-release methodology. We demonstrate that mature axons contain components of the endoplasmic reticulum and other biosynthetic organelles. Axonal organelles and sodium channel localization are sensitive to local blockade of the endoplasmic reticulum to Golgi transport. More importantly, secretory organelles are capable of delivering sodium channels to the plasma membrane in isolated axons, demonstrating an intrinsic capacity of the axonal biosynthetic route in regulating the axonal proteome in mammalian axons.en_US
Patrocinadordc.description.sponsorshipMejoramiento de la Calidad y la Equidad en la Educacion Superior (MECESUP), Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT), Iniciativa Cientifica Milenio (ICM)en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherNational Academy of Sciencesen_US
Type of licensedc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectRibosomesen_US
Keywordsdc.subjectLocalizationen_US
Keywordsdc.subjectTranslationen_US
Keywordsdc.subjectRanvieren_US
Keywordsdc.subjectNeuronsen_US
Keywordsdc.subjectTransporten_US
Keywordsdc.subjectMyelinated axonsen_US
Keywordsdc.subjectEndoplasmic-reticulumen_US
Keywordsdc.subjectMessenger-rnaen_US
Keywordsdc.subjectProtein-synthesisen_US
Títulodc.titleAxons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerveen_US
Document typedc.typeArtículo de revista


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile