The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles
Author
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Miguel De Pablos, Luis
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Diaz Lozano, Isabel María
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Jercic, María Isabel
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Quinzada, Markela
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Giménez, María José
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Calabuig, Eva
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Espino, Ana Margarita
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Gabriel Schijman, Alejandro
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Zulantay Alfaro, Inés
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Apt Baruch, Werner
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Osuna, Antonio
Admission date
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2016-12-19T20:32:16Z
Available date
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2016-12-19T20:32:16Z
Publication date
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2016
Cita de ítem
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Scientific Reports. Volumen: 6 Número de artículo: 27293
es_ES
Identifier
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10.1038/srep27293
Identifier
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https://repositorio.uchile.cl/handle/2250/141987
Abstract
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Trypanosoma cruzi is the etiological agent of Chagas disease, a neglected and emerging tropical disease, endemic to South America and present in non-endemic regions due to human migration. The MASP multigene family is specific to T. cruzi, accounting for 6% of the parasite's genome and plays a key role in immune evasion. A common feature of MASPs is the presence of two conserved regions: an N-terminal region codifying for signal peptide and a C-terminal (C-term) region, which potentially acts as GPI-addition signal peptide. Our aim was the analysis of the presence of an immune response against the MASP C-term region. We found that this region is highly conserved, released via exovesicles (EVs) and has an associated immune response as revealed by epitope affinity mapping, IFA and inhibition of the complement lysis assays. We also demonstrate the presence of a fast IgM response in Balb/c mice infected with T. cruzi. Our results reveal the presence of non-canonical secreted peptides in EVs, which can subsequently be exposed to the immune system with a potential role in evading immune system targets in the parasite.
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Patrocinador
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Fundacion Ramon Areces, Spanish Council of Science and Technology (CICyT), Eranet Lac Hid, Convocatoria de Incentivos a Proyectos de Excelencia Regional of Andalusian Government JJAA Project, Spanish Agency for International Cooperation and Development (AECID)