Expression of miR-22 and miR-150 in type 1 diabetes mellitus: Possible relationship with autoimmunity and clinical characteristics

Abstract

Background and objective: Type 1 diabetes (T1D) is an autoimmune disease of complex aetiology. Several microRNAs (miR) have been linked to the pathogenesis of autoimmune diseases.

To analyze the possible association of miR-22 and miR-150 with autoimmunity and clinical severity of T1D.

Patients and methods: The study was performed in peripheral blood mononuclear cells of 20 patients with T1D and 20 control subjects. The expression of miR-22 and miR-150 was performed in peripheral blood mononuclear cells using TaqMan probes to different glucose concentrations (baseline, 11 mm, 25 mm).

Results: Our results suggest that the expression of miR-22 is increased in T1D patients compared to the controls. This effect was observed in baseline glucose conditions and decreased in 11 and 25 mM of glucose. The expression of miR-150 was lower in T1D patients versus the controls. There was no correlation between the autoimmune profile and the two studied miRNAs. miR-22 (baseline condition) and miR-150 (11 mM condition) or the ketoacidosis component.

Conclusion: miR-22 and 150 were not associated with the autoimmune component present in T1D patients. (C) 2016 Elsevier Espana, S.L.U. All rights reserved.