Reversible redox modifications of ryanodine receptor ameliorate ventricular arrhythmias in the ischemic-reperfused heart
Author
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Becerra, Romina
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Román, Bárbara
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Di Carlo, Mariano
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Mariangelo, Juan Ignacio
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Salas, Margarita
Author
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Sánchez, Gina
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Donoso Laurent, Paulina
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Schinella, Guillermo
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Vittone, Leticia
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Wehrens, Xander
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Mundiña Weilenmann, Cecilia
Author
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Said, Matilde
Admission date
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2017-03-28T18:38:55Z
Available date
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2017-03-28T18:38:55Z
Publication date
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2016
Cita de ítem
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Am J Physiol Heart Circ Physiol 311: H713–H724, 2016.
es_ES
Identifier
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10.1152/ajpheart.00142.2016
Identifier
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https://repositorio.uchile.cl/handle/2250/143355
Abstract
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Previous results from our laboratory showed that phosphorylation of ryanodine receptor 2 (RyR2) by Ca2+ calmodulin-dependent kinase II (CaMKII) was a critical but not the unique event responsible for the production of reperfusion-induced arrhythmogenesis, suggesting the existence of other mechanisms cooperating in an additive way to produce these rhythm alterations. Oxidative stress is a prominent feature of ischemia/reperfusion injury. Both CaMKII and RyR2 are proteins susceptible to alteration by redox modifications. This study was designed to elucidate whether CaMKII and RyR2 redox changes occur during reperfusion and whether these changes are involved in the genesis of arrhythmias. Langendorff-perfused hearts from rats or transgenic mice with genetic ablation of CaMKII phosphorylation site on RyR2 (S2814A) were subjected to ischemia-reperfusion in the presence or absence of a free radical scavenger (mercaptopropionylglycine, MPG) or inhibitors of NADPH oxidase and nitric oxide synthase. Left ventricular contractile parameters and monophasic action potentials were recorded. Oxidation and phosphorylation of CaMKII and RyR2 were assessed. Increased oxidation of CaMKII during reperfusion had no consequences on the level of RyR2 phosphorylation. Avoiding the reperfusion-induced thiol oxidation of RyR2 with MPG produced a reduction in the number of arrhythmias and did not modify the contractile recovery. Conversely, selective prevention of S-nitrosylation and S-glutathionylation of RyR2 was associated with higher numbers of arrhythmias and impaired contractility. In S2814A mice, treatment with MPG further reduced the incidence of arrhythmias. Taken together, the results suggest that redox modification of RyR2 synergistically with CaMKII phosphorylation modulates reperfusion arrhythmias
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Patrocinador
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Consejo de Investigaciones Cientificas y Tecnicas (CONICET), Argentina (PIP) 0463
Agencia Nacional de Promocion Cientifica y Tecnica, Argentina (PICT) 2634 2073
PICT 0856
Fondecyt 1110257 1130407
NIH-NHLBI HL089598 HL091947 HL117641 HL129570
American Heart Association 13EIA14560061