Phylogenetic Analysis of Bolivian Bat Trypanosomes of the Subgenus Schizotrypanum Based on Cytochrome b Sequence and Minicircle Analyses
Author
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García, Lineth
Author
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Ortiz Zúñiga, Sylvia
Author
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Osorio Abarzúa, Carlos Gonzalo
Author
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Cruz Torrico, Mary
Author
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Torrico, Faustino
Author
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Solari Illescas, Aldo
Admission date
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2017-09-25T18:50:08Z
Available date
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2017-09-25T18:50:08Z
Publication date
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2012
Cita de ítem
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PLoS ONE May 2012 | Volume 7 | Issue 5 | e36578
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Identifier
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10.1371/journal.pone.0036578
Identifier
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https://repositorio.uchile.cl/handle/2250/145066
Abstract
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The aim of this study was to establish the phylogenetic relationships of trypanosomes present in blood samples of Bolivian
Carollia bats. Eighteen cloned stocks were isolated from 115 bats belonging to Carollia perspicillata (Phyllostomidae) from
three Amazonian areas of the Chapare Province of Bolivia and studied by xenodiagnosis using the vectors Rhodnius robustus
and Triatoma infestans (Trypanosoma cruzi marenkellei) or haemoculture (Trypanosoma dionisii). The PCR DNA amplified was
analyzed by nucleotide sequences of maxicircles encoding cytochrome b and by means of the molecular size of hyper
variable regions of minicircles. Ten samples were classified as Trypanosoma cruzi marinkellei and 8 samples as Trypanosoma
dionisii. The two species have a different molecular size profile with respect to the amplified regions of minicircles and also
with respect to Trypanosoma cruzi and Trypanosoma rangeli used for comparative purpose. We conclude the presence of
two species of bat trypanosomes in these samples, which can clearly be identified by the methods used in this study. The
presence of these trypanosomes in Amazonian bats is discussed.
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Patrocinador
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This study was funded by the European Community’s Seventh Framework Programme (FP7) under agreement 223034.