Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
Author
dc.contributor.author
Núñez, Harold
Author
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Ulloa Flores, María Teresa
Author
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Guerra, Fabiola
Author
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Osorio Abarzúa, Carlos Gonzalo
Admission date
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2017-09-28T18:43:24Z
Available date
dc.date.available
2017-09-28T18:43:24Z
Publication date
dc.date.issued
2009
Cita de ítem
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Rev Méd Chile 2009; 137: 208-214
es_ES
Identifier
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0034-9887
Identifier
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https://repositorio.uchile.cl/handle/2250/145109
Abstract
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Background: Most clinical isolates of Vibrio parahaemolyticus
produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is
encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most
environmental isolates are described as tdh negative. Aim: To assess if environmental strains
lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods:
Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI
was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes,
was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a
full PAI when compared with clinical strains. In environmental strains, Southern blot and
sequence analysis showed that a genetic region of 80 kilobase pairs including genes from
VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism
of Vibrio parahaemolyticus pathogenecity island region and suggest that new pathogenic
strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic
strains.