mTORC1 inhibitor rapamycin and ER stressor tunicamycin induce differential patterns of ER-mitochondria coupling
Author
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Bravo Sagua, Roberto
Author
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López Crisosto, Camila
Author
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Parra Ortíz, María Valentina
Author
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Rodríguez Peña, Marcelo
Author
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Rothermel, Beverly A.
Author
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Quest, Andrew F. G.
Author
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Lavandero González, Sergio
Admission date
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2017-11-14T18:40:17Z
Available date
dc.date.available
2017-11-14T18:40:17Z
Publication date
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2016
Cita de ítem
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Scientific Reports Vol. 6 No.de artículo: 36394 Nov 2016
es_ES
Identifier
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10.1038/srep36394
Identifier
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https://repositorio.uchile.cl/handle/2250/145622
Abstract
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Efficient mitochondrial Ca2+ uptake takes place at contact points between the ER and mitochondria, and represents a key regulator of many cell functions. In a previous study with HeLa cells, we showed that ER-to-mitochondria Ca2+ transfer increases during the early phase of ER stress induced by tunicamycin as an adaptive response to stimulate mitochondrial bioenergetics. It remains unknown whether other types of stress signals trigger similar responses. Here we observed that rapamycin, which inhibits the nutrient-sensing complex mTORC1, increased ER-mitochondria coupling in HeLa cells to a similar extent as did tunicamycin. Interestingly, although global responses to both stressors were comparable, there were notable differences in the spatial distribution of such changes. While tunicamycin increased organelle proximity primarily in the perinuclear region, rapamycin increased organelle contacts throughout the entire cell. These differences were paralleled by dissimilar alterations in the distribution of regulatory proteins of the ER-mitochondria interface, heterogeneities in mitochondrial Ca2+ uptake, and the formation of domains within the mitochondrial network with varying mitochondrial transmembrane potential. Collectively, these data suggest that while increasing ER-mitochondria coupling appears to represent a general response to cell stress, the intracellular distribution of the associated responses needs to be tailored to meet specific cellular requirements.