Aminochrome induces microglia and astrocyte activation
Author
dc.contributor.author
Santos, Cleonice C.
Author
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Araújo, Fillipe M.
Author
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Ferreira, Rafael S.
Author
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Silva, Vanessa B.
Author
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Silva, Juliana H.C.
Author
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Grangeiro, Maria S.
Author
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Soares, Érica N.
Author
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Pereira, Érica Patricia L.
Author
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Souza, Cleide S.
Author
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Costa, Silvia L.
Author
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Segura Aguilar, Juan
Author
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Silva, Victor Diogenes
Admission date
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2018-05-17T22:06:38Z
Available date
dc.date.available
2018-05-17T22:06:38Z
Publication date
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2017
Cita de ítem
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Toxicology in Vitro 42 (2017) 54–60
es_ES
Identifier
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10.1016/j.tiv.2017.04.004
Identifier
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https://repositorio.uchile.cl/handle/2250/147901
Abstract
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Aminochrome has been suggested as a more physiological preclinical model capable of inducing five of the six mechanisms of Parkinson's Disease (PD). Until now, there is no evidence that aminochrome induces glial activation related to neuroinflammation, an important mechanism involved in the loss of dopaminergic neurons. In this study, the potential role of aminochrome on glial activation was studied in primary mesencephalic neuron-glia cultures and microglial primary culture from Wistar rats. We demonstrated that aminochrome induced a reduction in the number of viable cells on cultures exposed to concentration between 10 and 100 M. Moreover, aminochrome induces neuronal death determined by Fluoro-jade B. Furthermore, we demonstrated that aminochrome induced reduction in the number of TH-immunoreactive neurons and reactive gliosis, featured by morphological changes in GFAP(+) and lba1(+) cells, increase in the number of OX-42(+) cells and increase in the number of NF-kappa B p50 immunoreactive cells. These results demonstrate aminochrome neuroinflammatory ability and support the hypothesis that it may be a better PD preclinical model to find new pharmacological treatment that stop the development of this disease.
es_ES
Patrocinador
dc.description.sponsorship
Coordenacao de Apoio de Pessoal de Nivel Superior, CAPES/PVE - 189576/09-2014 /
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico do Brasil, CNPq - 470807/2011-3 /
Fundacao de Apoio a Pesquisa do Estado da Bahia, FAPESB RED0004/2011