Show simple item record

Authordc.contributor.authorGarcía González, Paulina 
Authordc.contributor.authorSchinnerling, Katina 
Authordc.contributor.authorSepúlveda Gutierrez, Alejandro 
Authordc.contributor.authorMaggi, Jaxaira 
Authordc.contributor.authorMehdi, Ahmed M. 
Authordc.contributor.authorNel, Hendrik J. 
Authordc.contributor.authorPesce Reyes, Bárbara 
Authordc.contributor.authorLarrondo Lillo, Milton 
Authordc.contributor.authorAravena, Octavio 
Authordc.contributor.authorMolina Sampayo, María Carmen 
Authordc.contributor.authorCatalán Martina, Diego 
Authordc.contributor.authorThomas, Ranjeny 
Authordc.contributor.authorVerdugo Salgado, Ricardo 
Authordc.contributor.authorAguillón Gutiérrez, Juan Carlos 
Cita de ítemdc.identifier.citationFront. Immunol. 8:1350es_ES
Abstractdc.description.abstractThere is growing interest in the use of tolerogenic dendritic cells (tolDCs) as a potential target for immunotherapy. However, the molecular bases that drive the differentiation of monocyte-derived DCs (moDCs) toward a tolerogenic state are still poorly understood. Here, we studied the transcriptional profile of moDCs from healthy subjects, modulated with dexamethasone (Dex) and activated with monophosphoryl lipid A (MPLA), referred to as Dex-modulated and MPLA-activated DCs (DM-DCs), as an approach to identify molecular regulators and pathways associated with the induction of tolerogenic properties in tolDCs. We found that DM-DCs exhibit a distinctive transcriptional profile compared to untreated (DCs) and MPLA-matured DCs. Differentially expressed genes downregulated by DM included MMP12, CD1c, IL-1B, and FCER1A involved in DC maturation/inflammation and genes upregulated by DM included JAG1, MERTK, IL-10, and IDO1 involved in tolerance. Genes related to chemotactic responses, cell-to-cell signaling and interaction, fatty acid oxidation, metal homeostasis, and free radical scavenging were strongly enriched, predicting the activation of alternative metabolic processes than those driven by counterpart DCs. Furthermore, we identified a set of genes that were regulated exclusively by the combined action of Dex and MPLA, which are mainly involved in the control of zinc homeostasis and reactive oxygen species production. These data further support the important role of metabolic processes on the control of the DC-driven regulatory immune response. Thus, Dex and MPLA treatments modify gene expression in moDCs by inducing a particular transcriptional profile characterized by the activation of tolerance-associated genes and suppression of the expression of inflammatory genes, conferring the potential to exert regulatory functions and immune response modulation.es_ES
Patrocinadordc.description.sponsorshipFondecyt-Chile 1140553 Millenium Institute on Immunology and Immunotherapy P09/016-Fes_ES
Publisherdc.publisherFrontiers Media SAes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers In Immunologyes_ES
Keywordsdc.subjectTolerogenic dendritic cellses_ES
Keywordsdc.subjectImmune regulationes_ES
Keywordsdc.subjectTolerance inductiones_ES
Títulodc.titleDexamethasone and monophosphoryl lipid a induce a distinctive profile on monocyte-derived dendritic cells through transcriptional modulation of genes associated with essential processes of the immune responsees_ES
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación ISIes_ES

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile