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Authordc.contributor.authorValladares, Macarena 
Authordc.contributor.authorPlaza Parrochia, Francisca 
Authordc.contributor.authorLepez, Macarena 
Authordc.contributor.authorLópez Ponce, Daniela 
Authordc.contributor.authorGabler Neale, Fernando 
Authordc.contributor.authorGayan, Patricio 
Authordc.contributor.authorSelman, Alberto 
Authordc.contributor.authorVega Blanco, María Margarita 
Authordc.contributor.authorRomero Osses, Carmen 
Cita de ítemdc.identifier.citationHistol Histopathol 32, (11) 1187-1196es_ES
Abstractdc.description.abstractIntroduction: Ovarian cancer presents a high angiogenesis (formation of new blood vessels) regulated by pro-angiogenic factors, mainly vascular endothelial growth factor (VEGF) and nerve growth factor (NGF). An association between endogenous levels of estrogen and increased risk of developing ovarian cancer has been reported. Estrogen action is mediated by the binding to its specific receptors (ER alpha and ER beta), altered ER alpha/ER beta ratio may constitute a marker of ovarian carcinogenesis progression. Objective: To determine the effect of estradiol through ERa on the expression of NGF and VEGF in epithelial ovarian cancer (EOC). Methodology: Levels of phosphorylated estrogen receptor alpha (pERa) were evaluated in well, moderate and poorly differentiated EOC samples (EOC-I, EOC-II, EOC-III). Additionally, ovarian cancer explants were stimulated with NGF (0, 10 and 100 ng/ml) and ER alpha, ER beta and pERa levels were detected. Finally, human ovarian surface epithelial (HOSE) and epithelial ovarian cancer (A2780) cell lines were stimulated with estradiol, where NGF and VEGF protein levels were evaluated. Results: In tissues, ERs were detected being pERa levels significantly increased in EOC-III samples compared with EOC-I (p< 0.05). Additionally, ovarian explants treated with NGF increased pERa levels meanwhile total ER alpha and ER beta levels did not change. Cell lines stimulated with estradiol revealed an increase of NGF and VEGF protein levels (p< 0.05). Conclusions: Estradiol has a positive effect on pro-angiogenic factors such as NGF and VEGF expression in EOC, probably through the activation of ER alpha; generating a positive loop induced by NGF increasing pERa levels in epithelial ovarian cells.es_ES
Patrocinadordc.description.sponsorshipFondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) 1110372 1160139es_ES
Publisherdc.publisherFrancisco Hernández, Editores_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceHistology and histopathologyes_ES
Keywordsdc.subjectEpithelial Ovarian canceres_ES
Keywordsdc.subjectEstradiol Receptorses_ES
Títulodc.titleEffect of estradiol on the expression of angiogenic factors in epithelial ovarian canceres_ES
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile