Contribution of riboflavin supply pathways to Vibrio cholerae in different environments
Author
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Fuentes Flores, Andrés
Author
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Sepúlveda Cisternas, Ignacio
Author
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Vásquez Solis de Ovando, José Ignacio
Author
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Torres, Alexia
Author
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García Angulo, Victor
Admission date
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2018-06-15T19:55:09Z
Available date
dc.date.available
2018-06-15T19:55:09Z
Publication date
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2017
Cita de ítem
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Gut Pathog (2017) 9: 64
es_ES
Identifier
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10.1186/s13099-017-0214-9
Identifier
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https://repositorio.uchile.cl/handle/2250/148913
Abstract
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Background: The waterborne diarrheagenic bacterium Vibrio cholerae, cause of the pandemic cholera disease, thrives in a variety of environments ranging from estuarine waters to the human intestinal tract. This species has two ways to obtain the essential micronutrient riboflavin, de novo biosynthesis and environmental uptake through the RibN importer. The way these functions interrelate to fulfill riboflavin needs in different conditions in this species is unknown.
Results: This study analyzed the contributions of riboflavin biosynthesis and transport to the culturability of Vibrio cholerae in river and seawater in vitro and in the Caenorhabditis elegans nematode host model. Elimination of the ribD riboflavin biosynthetic gene renders the bacteria riboflavin-auxotrophic, while a ribN mutant strain has no growth defect in minimal media. When growing in river water, deletion of ribD causes an impairment in culturability. In this condition, the Delta ribN strain has a defect to compete against a wild type strain but outcompetes the Delta ribD strain. The latter effect is inverted by the addition of riboflavin to the water. In contrast, growth in seawater causes a loss in culturability independent of riboflavin biosynthesis or transport. In the C. elegans model, only the Delta ribD strain is attenuated.
Conclusion: Results indicate that while riboflavin biosynthesis seems to outweigh riboflavin uptake, the latter may still provide a selective advantage to V. cholerae in some environments.