Exploring antioxidant reactivity and molecular structure of phenols by means of two coupled assays using fluorescence probe (2,3-diazabicyclo[2.2.2]oct-2-ene, DBO) and free radical (2,2-diphenyl-1-picrylhydrazyl, DPPH )
Author
dc.contributor.author
Becerra Herrera, Mercedes
Author
dc.contributor.author
Sayago, Ana
Author
dc.contributor.author
Beltran, Rafael
Admission date
dc.date.accessioned
2018-06-25T13:45:42Z
Available date
dc.date.available
2018-06-25T13:45:42Z
Publication date
dc.date.issued
2017
Cita de ítem
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J. Chem. Sci. Vol. 129, No. 9, September 2017, pp. 1381–1390
es_ES
Identifier
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10.1007/s12039-017-1331-1
Identifier
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https://repositorio.uchile.cl/handle/2250/149167
Abstract
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Phenolic compounds can be considered as the most important bioactive compounds in Mediterranean diet. However, many of the complex connections between phenols antioxidant reactivity and their molecular structure remain unsolved. To shine light on these issues, the antioxidant reactivity of 15 relevant phenolic compounds was studied. Two different analytical approaches were combined: (a) the well-established 2,2-diphenyl-1-picrylhydrazyl ) stable free radical assay, and (b) the 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) fluorescent probe assay. The results obtained confirm how certain structural features (i.e., hydroxyl and methoxy groups, alkyl and alkenyl chains) play a critical role in the singular antioxidant response exhibited by each phenolic compound. This knowledge provides decisive information to select a specific phenolic compound as an antioxidant additive or for the chemical design of new antioxidants.
Synopsis (for Contents page): Interactions between DBO (fluorescence quenching) and DPPH (radical scavenging) with fifteen phenolic compounds, which are present in olive oil and wine, served as a measure of antioxidant activity of the phenols and correlated to the molecular structure.
es_ES
Patrocinador
dc.description.sponsorship
Environmental Council of the Andalusia Regional Government
P10 FQM 6185
Operative Program of Transborderline Cooperation Spain-Portugal
0434_I2TEP_5_E OLITRACE
FONDECYT
3150059
Exploring antioxidant reactivity and molecular structure of phenols by means of two coupled assays using fluorescence probe (2,3-diazabicyclo[2.2.2]oct-2-ene, DBO) and free radical (2,2-diphenyl-1-picrylhydrazyl, DPPH )