Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
Author
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Rodrigues Marcondes, Rodrigo
Author
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Cândido Carvalho, Katia
Author
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Giannocco, Gisele
Author
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Coelho Duarte, Daniele
Author
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Garcia, Natalia
Author
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Soares Junior, Jose Maria
Author
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Cotrim Guerreiro da Silva, Ismael Dale
Author
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Maliqueo Yevilao, Manuel
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Chada Baracat, Edmun
Author
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Gustavo Arantes, Rosa Maciel
Admission date
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2018-06-29T15:16:33Z
Available date
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2018-06-29T15:16:33Z
Publication date
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2017
Cita de ítem
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Clinics 2017; 72(8): 510-514
es_ES
Identifier
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10.6061/clinics/2017(08)09
Identifier
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https://repositorio.uchile.cl/handle/2250/149359
Abstract
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OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol.
METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis.
RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor.
CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats.
es_ES
Patrocinador
dc.description.sponsorship
Fundacao de Amparo Pesquisa do Estado de Sao Paulo (FAPESP) -Brazil
2010/17417-3
2013/12830-8
Master's degree scholarship Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) -Brazil
134694/2010-4
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes