Characterization of microsatellite markers for Broussonetia papyrifera (Moraceae)
Author
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Penailillo, Johany
Author
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Kuo, Wenhsi
Author
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Ceballos Olivares, Gabriela Paz
Author
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Silva Poblete, Gerardo
Author
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Peña Ahumada, Bárbara
Author
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Muñoz, Sofía
Author
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Moncada, Ximena
Author
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Chung, Kuo Fang
Author
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Seelenfreund Hirsch, Daniela
Author
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Seelenfreund, Andrea
Admission date
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2018-07-11T19:45:44Z
Available date
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2018-07-11T19:45:44Z
Publication date
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2017
Cita de ítem
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Applications in Plant Sciences, 2017 5(8): 1700044
es_ES
Identifier
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10.3732/apps.1700044
Identifier
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https://repositorio.uchile.cl/handle/2250/149757
Abstract
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Premise of the study: Broussonetia papyrifera (Moraceae) is native to Asia and is used as a medicinal plant and as a source of fiber for making paper. It was dispersed into the Pacific region as a fiber source for making nonwoven textiles (barkcloth). Microsatellites were developed to trace the human-mediated dispersal of this species into the Pacific region.
Methods and Results: A set of 36 microsatellites was isolated and initially assayed on 10 accessions to assess polymorphism. We found that 20 markers were polymorphic, with the number of alleles per marker ranging from four to 35 in 70 accessions genotyped from three Asian populations. Observed and expected heterozygosities ranged from 0.04 to 0.85 and from 0.19 to 0.94, respectively. These markers were tested in four Moraceae species and one Rosaceae species.
Conclusions: These markers will be useful for the assessment of genetic diversity in B. papyrifera. They show low transferability to other species tested.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) from Chile
1080061
1120175
Biodiversity Research Center, Academia Sinica, Taiwan