Respiratory syncytial virus genotypes, host immune profiles, and disease severity in young children hospitalized with bronchiolitis
Author
dc.contributor.author
Rodríguez Fernández, Rosa
Author
dc.contributor.author
Tapia Faúndes, Lorena
Author
dc.contributor.author
Yang, Chin Fen
Author
dc.contributor.author
Torres Torretti, Juan Pablo
Author
dc.contributor.author
Chavez Bueno, Susana
Author
dc.contributor.author
García, Carla
Author
dc.contributor.author
Jaramillo, Lisa M.
Author
dc.contributor.author
Moore Clingenpeel, Melissa
Author
dc.contributor.author
Jafri, Hasan S.
Author
dc.contributor.author
Peeples, Mark E.
Author
dc.contributor.author
Piedra, Pedro A.
Author
dc.contributor.author
Ramilo, Octavio
Author
dc.contributor.author
Mejias, Asuncion
Admission date
dc.date.accessioned
2018-07-26T15:02:30Z
Available date
dc.date.available
2018-07-26T15:02:30Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Journal of Infectious Diseases 2018; 217: 24–34
es_ES
Identifier
dc.identifier.other
10.1093/infdis/jix543
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/150303
Abstract
dc.description.abstract
Background. Data on how respiratory syncytial virus (RSV) genotypes influence disease severity and host immune responses is limited. Here, we characterized the genetic variability of RSV during 5 seasons, and evaluated the role of RSV subtypes, genotypes, and viral loads in disease severity and host transcriptional profiles.
Methods. A prospective, observational study was carried out, including a convenience sample of healthy infants hospitalized with RSV bronchiolitis. Nasopharyngeal samples for viral load quantitation, typing, and genotyping, and blood samples for transcriptome analyses were obtained within 24 hours of hospitalization. Multivariate models were constructed to identify virologic and clinical variables predictive of clinical outcomes.
Results. We enrolled 253 infants (median age 2.1 [25%-75% interquartile range] months). RSV A infections predominated over RSV B and showed greater genotype variability. RSV A/GA2, A/GA5, and RSV B/BA were the most common genotypes identified. Compared to GA2 or BA, infants with GA5 infections had higher viral loads. GA5 infections were associated with longer hospital stay, and with less activation of interferon and increased overexpression of neutrophil genes.
Conclusions. RSV A infections were more frequent than RSV B, and displayed greater variability. GA5 infections were associated with enhanced disease severity and distinct host immune responses.