Heparan sulfate potentiates leukocyte adhesion on cardiac fibroblast by enhancing Vcam-1 and Icam-1 expression
Author
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Olivares Silva, Francisco Javier
Author
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Landaeta, Rodolfo
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Aránguiz, Pablo
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Bolivar, Samir
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Humeres Martínez, Claudio
Author
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Anfossi, Renatto
Author
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Vivar Sánchez, Raúl
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Boza Fuentes, Pía
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Muñoz Jofré, Claudia
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Pardo Jiménez, Viviana Gladys
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Peiro, Concepción
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Sánchez Ferrer, Carlos
Author
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Díaz Araya, Guillermo
Admission date
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2018-07-30T15:43:56Z
Available date
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2018-07-30T15:43:56Z
Publication date
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2018
Cita de ítem
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BBA - Molecular Basis of Disease, 1864 (2018): 831–842
es_ES
Identifier
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10.1016/j.bbadis.2017.12.002
Identifier
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https://repositorio.uchile.cl/handle/2250/150425
Abstract
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Cardiac fibroblasts (CF) act as sentinel cells responding to chemokines, cytokines and growth factors released in cardiac tissue in cardiac injury events, such as myocardial infarction (MI). Cardiac injury involves the release of various damage-associated molecular patterns (DAMPs) including heparan sulfate (HS), a constituent of the extracellular matrix (ECM), through the TLR4 receptor activation triggering a strong inflammatory response, inducing leukocytes recruitment. This latter cells are responsible of clearing cell debris and releasing cytokines that promote CF differentiation to myofibroblast (CMF), thus initiating scar formation.
CF were isolated from adult male rats and subsequently stimulated with HS or LPS, in the presence or absence of chemical inhibitors, to evaluate signaling pathways involved in ICAM-1 and VCAM-1 expression. siRNA against ICAM-1 and VCAM-1 were used to evaluate participation of these adhesion molecules on leukocytes recruitment.
HS through TLR4, PI3K/Ala and NF-KB increased ICAM-1 and VCAM-1 expression, which favored the adhesion of spleen mononuclear cells (SMC) and bone marrow granulocytes (PMN) to CF. These effects were prevented by siRNA against ICAM-1 and VCAM-1. Co-culture of CF with SMC increased alpha-SMA expression, skewing CF towards a pro-fibrotic phenotype, while CF pretreatment with HS partially reverted this effect.
Conclusion: These data show the dual role of HS during the initial stages of wound healing. Initially, HS enhance the pro-inflammatory role of CF increasing cytokines secretion; and later, by increasing protein adhesion molecules allows the adhesion of SMC on CF, which trigger CF-to-CMF differentiation.