Interactome screening identifies the ER luminal chaperone Hsp47 as a regulator of the unfolded protein response transducer IRE1 alpha
Author
dc.contributor.author
Sepúlveda, Denisse
Author
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Rojas Rivera, Diego
Author
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Rodríguez, Diego
Author
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Kohler, Andrés
Author
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Urra, Hery
Author
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Carreras Sureda, Amado
Author
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Hazari, Younis
Author
dc.contributor.author
Sierralta, Jimena
Author
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Hetz Flores, Claudio
Admission date
dc.date.accessioned
2018-07-30T16:54:44Z
Available date
dc.date.available
2018-07-30T16:54:44Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Molecular Cell 69, 238–252 (2018)
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Identifier
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10.1016/j.molcel.2017.12.028
Identifier
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https://repositorio.uchile.cl/handle/2250/150446
Abstract
dc.description.abstract
Maintenance of endoplasmic reticulum (ER) proteostasis is controlled by a dynamic signaling network known as the unfolded protein response (UPR). IRE1a is a major UPR transducer, determining cell fate under ER stress. We used an interactome screening to unveil several regulators of the UPR, highlighting the ER chaperone Hsp47 as the major hit. Cellular and biochemical analysis indicated that Hsp47 instigates IRE1 alpha signaling through a physical interaction. Hsp47 directly binds to the ER luminal domain of IRE1 alpha with high affinity, displacing the negative regulator BiP from the complex to facilitate IRE1 alpha oligomerization. The regulation of IRE1 alpha signaling by Hsp47 is evolutionarily conserved as validated using fly and mouse models of ER stress. Hsp47 deficiency sensitized cells and animals to experimental ER stress, revealing the significance of Hsp47 to global proteostasis maintenance. We conclude that Hsp47 adjusts IRE1 alpha signaling by fine-tuning the threshold to engage an adaptive UPR.
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Patrocinador
dc.description.sponsorship
FONDECYT
1140549
3130365
3150113
3160461
FONDAP program
15150012
Millennium Institute
P09-015-F
European Commission RD MSCA-RISE
734749
Michael J. Fox Foundation for Parkinson's Research Target Validation grant
9277
FONDEF
ID16I10223
D11E1007
US Office of Naval Research Global
N62909-16-1-2003
U.S. Air Force Office of Scientific Research
FA9550-16-1-0384
ALSRP Therapeutic Idea Award
AL150111
Muscular Dystrophy Association
382453
CONICYT-Brazil
441921/2016-7
CONICYT Ph.D. fellowship
21130169
Canadian Institutes of Health research grants
MOP-15291
MOP-15415
MOP-53050
INSERM
Societe Francophone du Diabete (SFD/MSD)
Societe Francaise d'Hepatologie (AFEF/Aptalis)
La Ligue contre le Cancer (BMM)
French government (National Research Agency, ANR) "Investments for the Future'' LABEX SIGNALIFE
ANR-11-LABX-002801
German Research Foundation (DFG)
GO 1987/2-1
BNI-P09015F
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Lenguage
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en
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Publisher
dc.publisher
Cell Press
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Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States