Progression of type 2 helper T celletype inflammation and airway remodeling in a rodent model of naturally acquired subclinical primary pneumocystis infection
Author
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Iturra, Pablo
Author
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Rojas, Diego A.
Author
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Pérez, Francisco J.
Author
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Méndez, Andrea
Author
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Ponce, Carolina A.
Author
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Bonilla, Paula
Author
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Bustamante, Rebeca
Author
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Rodríguez, Héctor
Author
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Beltrán, Caroll J.
Author
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Vargas, Sergio L.
Admission date
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2018-07-31T15:39:33Z
Available date
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2018-07-31T15:39:33Z
Publication date
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2018
Cita de ítem
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The American Journal of Pathology Vol. 188, No. 2, February 2018
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Identifier
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10.1016/j.ajpath.2017.10.019
Identifier
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https://repositorio.uchile.cl/handle/2250/150488
Abstract
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Subclinical primary Pneumocystisinfection is the most common pulmonary infection in early infancy, making
it important to determine whether it damages the lung. Pneumocystis peaks at 2 to 5 months of age, when
respiratory morbidity coincidently increases. We have documented that Pneumocystis increases mucus
production in infant lungs, and animal models reveal lung lesions that warrant characterization. Herein,
immunocompetent rats infected at birth with Pneumocystis by cohabitation, to resemble communityacquired
infection, underwent lung assessments at 45, 60, and 75 days of age. Lungs fixed by vascular
perfusion to prevent collapse during necropsy were used for morphometry evaluations of mucus production,
airway epithelial thickening, perivascular and peribronchiolar inflammation, and structural airway remodeling.
Changes in these histologic features indicate lung disease. Selected immune markers were assessed in
parallel using fresh-frozen lung tissue from sibling rats of the same cages. Sequential activation of NF-kB and
an increased Gata3/T-bet mRNA level ratio, consistent with a type 2 helper T-celletype inflammatory
response, and subacute fibrosis were recognized. Therefore, documenting subclinical Pneumocystisinfection
induces lung disease in the immunocompetent host. Taken together with the peak age of primary Pneumocystisinfection,
results warrant investigating the clinical impact of this often subclinical infection on the
severity of respiratory diseases in early infancy. This model can also be used to assess the effects of airway
insults, including coinfections by recognized respiratory pathogens. (Am J Pathol 2018, 188: 417e431;
https://doi.org/10.1016/j.ajpath.2017.10.019)
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Patrocinador
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e Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT-Chile) grants 1100225 and 1140412 (S.L.V.), Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) under ERANet LAC grant ELAC2014/HID-0254 (S.L.V.), FONDECYT-Chile Postdoctoral Fellow grant 3140391 (D.A.R.), and the Chilean Doctoral Scholarship Fund (P.A.I., F.J.P., and A.M.)
Progression of type 2 helper T celletype inflammation and airway remodeling in a rodent model of naturally acquired subclinical primary pneumocystis infection