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Authordc.contributor.authorCaviedes Codelia, Raúl 
Authordc.contributor.authorLiberona Leppe, José 
Authordc.contributor.authorHidalgo Tapia, Jorge 
Authordc.contributor.authorTascón, Soledad 
Authordc.contributor.authorSalas, Karime 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Cita de ítemdc.identifier.citationBiochimica et Biophysica Acta Volumen: 1134 Número: 3 Páginas: 247-255 apr 1992es_ES
Abstractdc.description.abstractA cell line (RCMH) in permanent culture was established from surgically removed adult normal human skeletal muscle by exposure to conditioned media obtained from thyroid cells. Cells proliferated indefinitely but displayed density inhibition of growth while maintaining some differentiated markers. Under certain incubation conditions, cells fused into myotube-like structures, with a concomitant increase in muscle specific proteins, such as human myoglobin, skeletal muscle myosin, desmin and dystrophin, as identified using immunocytochemical procedures. In addition, RCMH cells displayed high affinity receptors for alpha-bungarotoxin (B(max) = 0.7 pmol/mg protein, K(d) = 1.5 nM) and dihydropyridines (B(max) = 0.3 pmol/mg protein, K(d) = 0.5 nM for [H-3]PN200-110); these values are comparable to those reported for muscle cells in primary culture. Patch-clamp studies showed the presence of 42 pS carbachol gated channels and of 5 pS calcium channels (current carried by barium); chloride and potassium channels were also seen. This new cell line appears to bc a convenient model system to study skeletal muscle function.es_ES
Patrocinadordc.description.sponsorshipNIGMS NIH HHS GM 35981es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceBiochimica et Biophysica Actaes_ES
Keywordsdc.subjectSkeletal musclees_ES
Keywordsdc.subjectCell linees_ES
Keywordsdc.subjectDihydropyridine receptores_ES
Keywordsdc.subjectAlpha-bungarotoxin receptores_ES
Keywordsdc.subjectIon channeles_ES
Títulodc.titleA human skeletal-muscle cell-line obtained from an adult donores_ES
Document typedc.typeArtículo de revistaes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile