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Authordc.contributor.authorJaimovich Pérez, Enrique 
Authordc.contributor.authorDonoso Laurent, Paulina 
Authordc.contributor.authorLiberona Leppe, José 
Authordc.contributor.authorHidalgo Tapia, María Cecilia 
Admission datedc.date.accessioned2018-08-29T14:42:23Z
Available datedc.date.available2018-08-29T14:42:23Z
Publication datedc.date.issued1986
Cita de ítemdc.identifier.citationBioehimica et Biophysica Acta 855 (1986) 89-98es_ES
Identifierdc.identifier.other10.1016/0005-2736(86)90192-6
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/151356
Abstractdc.description.abstractThe presence of four cation pathways in membrane vesicles isolated from transverse tubules of frog and rabbit skeletal muscle was studied by measuring binding of specific blockers. Transverse tubules purified from frog muscle have a maximal binding capacity for [3H]nitrendipine (a marker for voltage-dependent calcium channels) of 130 pmol/mg of protein; this binding is strongly dependent on temperature and, at 37°C, on the presence of diltiazem. Receptors for [3Hlethylenediamine tetrodotoxin (a marker for voltagedependent sodium channels) and for 12SI-labeled a-bungarotoxin (a marker for acetylcholine-mediated channels) showed maximal binding values of about 5 pmol/mg. The number of sodium-pumping sites in the isolated tubule vesicles, inferred from [3H]ouabain binding, was 215 pmol/mg.'The high purity of this preparation makes feasible the use of these values as a criterion to judge the degree of purity of isolated preparations, and it allows investigation of transverse tubule contamination in other muscle membrane fractions.es_ES
Patrocinadordc.description.sponsorshipNHLBI NIH HHS HL 23007es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherElsevieres_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceBioehimica et Biophysica Actaes_ES
Keywordsdc.subjectTransverse tubulees_ES
Keywordsdc.subjectTetrodotoxines_ES
Keywordsdc.subjectDihydropyridinees_ES
Keywordsdc.subjectNa+ transportes_ES
Keywordsdc.subjectCa2+ transportes_ES
Keywordsdc.subjectBungarotoxines_ES
Keywordsdc.subjectFrog skeletal musclees_ES
Keywordsdc.subjectRabbit skeletal musclees_ES
Títulodc.titleIon pathways in transverse tubules. Quantification of receptors in membranes isolated from frog and rabbit skeletal musclees_ES
Document typedc.typeArtículo de revistaes_ES
Catalogueruchile.catalogadorlajes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile