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Authordc.contributor.authorLardone, M. C. 
Authordc.contributor.authorArgandoña, F. 
Authordc.contributor.authorLorca, M. 
Authordc.contributor.authorPiottante, A. 
Authordc.contributor.authorPalma Ceppi, Cristian 
Authordc.contributor.authorEbensperger, Mauricio 
Authordc.contributor.authorCastro, A. 
Authordc.contributor.authorFlorez, M. 
Admission datedc.date.accessioned2018-09-03T18:37:41Z
Available datedc.date.available2018-09-03T18:37:41Z
Publication datedc.date.issued2018-04
Cita de ítemdc.identifier.citationMolecular Human Reproduction, Vol.24, No.4 pp. 203–210, 2018es_ES
Identifierdc.identifier.other10.1093/molehr/gay006
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/151449
Abstractdc.description.abstractSTUDY QUESTION: Is the expression of steroidogenic enzyme 17 alpha-Hydroxylase/17,20-Lyase (CYP17A1) down-regulated in Leydig cells (LCs) of men with spermatogenic failure and compensated impairment of LC function, i.e. a low testosterone to LH (T/LH) ratio? SUMMARY ANSWER: Although the transcriptional expression of CYP17A1 is increased, its protein expression is decreased, in isolated LCs of men with spermatogenic failure and reduced serum T/LH. WHAT IS KNOWN ALREADY: Primary spermatogenic defects have been associated with functional and morphological abnormalities of LCs, characterized by decreased serum testosterone (T) levels, decreased T/LH, increased 17 beta-estradiol (E2) and E2/T ratio, and larger clusters of LCs (LC hyperplasia). CYP17A1 is a key enzyme in the testosterone pathway and has been implicated in the steroidogenic lesion produced by E2 stimulation. STUDY DESIGN, SIZE, DURATION: We studied 18 azoospermic patients with Sertoli cell-only syndrome (SCOS) and signs of LC dysfunction (cases) and 10 obstructive azoospermic/oligozoospermic men with normal spermatogenesis (controls). The SCOS patients were sub-grouped into 9 cases with T/LH <2 and 9 cases with T/LH >= 2. All of the men underwent testicular biopsy for sperm retrieval at the Reproductive Unit of a University Hospital. PARTICIPANTS/MATERIALS, SETTING, METHODS: The transcriptional expression of CYP17A1 and SF-1 (steroidogenic factor 1) was quantified by SYBR (R) Green-based qPCR in LCs isolated by laser capture microdissection (LCM), and relative expression to the control pool was assessed. CYP17A1 protein expression was semi-quantified by indirect immunofluorescence (IFI) using Image-Pro Plus v7.0 (Media Cybernetics) in testicular tissue. FSH and LH serum concentrations, and serum and intratesticular T (ITT) and E2 (ITE2) were measured by IRMA and RIA, respectively. MAIN RESULTS AND THE ROLE OF CHANCE: Relative CYP17A1 mRNA expression was increased in cases with T/LH <2 compared to cases with T/LH >= 2, by a mean of 3.3-fold (P = 0.002). No corresponding increase in protein expression was found; in fact, CYP17A1 immunostaining intensity assessed by the Integrated Optical Density (IOD) parameter was lower in the cases with T/LH <2 compared to controls (P = 0.008). Relative SF-1 mRNA expression was similar in both case subgroups. CYP17A1 mRNA expression correlated with ITE2 and intratesticular E2/T (r = 0.536; P = 0.026 and r = 0.542; P = 0.016, respectively), while an inverse association was observed for ITE2 and protein level expression (r = -0.421; P = 0.05). LARGE SCALE DATA: Not applicable. LIMITATIONS REASONS FOR CAUTION: We should interpret the results of the semi-quantification of immunofluorescent staining by Image-Pro Plus software with caution, because it is a semi-quantitative method that may have certain difficulties regarding the disposition of protein in the cells. However, it is not influenced by variations in the number of cells that express the protein, as could be the case of western blot analysis in testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: Dysfunctional LCs of men with SCOS show post-transcriptional deregulation of CYP17A1, with increased mRNA and decreased protein expression, which may be modulated by increased ITE2 levels. In addition, transcriptional expression of CYP17A1 was not associated with changes in SF-1 mRNA expression.es_ES
Patrocinadordc.description.sponsorshipNational Fund for Scientific and Technological Development (FONDECYT) of Chile 1120176es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherOxford Univ. Presses_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Sourcedc.sourceMolecular Human Reproductiones_ES
Keywordsdc.subjectSpermatogenic failurees_ES
Keywordsdc.subjectLeydig cell dysfunctiones_ES
Keywordsdc.subjectCYP17A1es_ES
Keywordsdc.subjectSertoli cell-only syndromees_ES
Keywordsdc.subjectSteroidogenic factor 1es_ES
Títulodc.titleLeydig cell dysfunction is associated with post-transcriptional deregulation of CYP17A1 in men with sertoli cell-only syndromees_ES
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorrgfes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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