Tetrodotoxin-sensitive sodium channels in a continuously cultured cell line derived from the adult rat cerebellum
Author
dc.contributor.author
Caviedes, R.
Author
dc.contributor.author
Díaz, M. A.
Author
dc.contributor.author
Compagnon, D.
Author
dc.contributor.author
Liberona Leppe, José
Author
dc.contributor.author
Cury, M.
Author
dc.contributor.author
Jaimovich Pérez, Enrique
Admission date
dc.date.accessioned
2018-09-11T20:33:28Z
Available date
dc.date.available
2018-09-11T20:33:28Z
Publication date
dc.date.issued
1986
Cita de ítem
dc.identifier.citation
Brain Research, 365 (1986) 259-268
es_ES
Identifier
dc.identifier.other
10.1016/0006-8993(86)91637-9
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/151625
Abstract
dc.description.abstract
A method to establish continuously cultured cell lines from adult cerebellar cortex is reported. Clones (prepared by this procedure)
were isolated from cerebellar established cultures at the 25th passage and after 15 months in vitro. One clone (UCHCC1) was mainrained
in culture and studied while the others were frozen. The cerebellar cell line UCHCC1 retained a neuronal-like morphology; the
addition of dimethylsulfoxide (DMSO) to the culture medium elicited a reproducible morphological 'differentiation' event, characterized
mainly by process extension. In 'differentiated' cells, veratridine significantly increased the uptake of 22Na. Such enhanced uptake
was blocked by tetrodotoxin (TFX) with a half-maximal inhibitory concentration of 0.9 nM. Binding of an [3H]ethylenediamine
derivative of TTX ([3H]en-TTX) to the microsomal fraction prepared from same DMSO-treated cells, showed a single class of receptors
with a maximal binding (Bmax) of 173 fmol/mg protein and a K d of 1.1 nM. Thyroid UCHT1 cells and 'undifferentiated' (cultured
without DMSO) cerebellar cells, did not show significant effects of veratridine on 22Na-uptake, or [3H]en-TFX binding. The 'differentiated'
nerve-like properties, induced by appropriate environmental manipulation, demonstrate the usefulness of cerebeUar UCHCC1
cells as a model system for the developing central neuron. On the other hand, the novel transforming procedure opens new possibilities
for obtaining permanent cell lines from other regions of the adult CNS.