A regulatory RNA is involved in RNA duplex formation and biofilm regulation in sulfolobus acidocaldarius
Author
dc.contributor.author
Orell, Alvaro
Author
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Tripp, Vanessa
Author
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Aliaga-Tobar, Victor
Author
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Albers, Sonja-Verena
Author
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Maracaja Coutinho, Vinicius
Author
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Randau, Lennart
Admission date
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2018-10-02T16:45:35Z
Available date
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2018-10-02T16:45:35Z
Publication date
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2018-05-18
Cita de ítem
dc.identifier.citation
Nucleic Acids Research, 2018, Vol. 46, No. 9
es_ES
Identifier
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10.1093/nar/gky144
Identifier
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https://repositorio.uchile.cl/handle/2250/151917
Abstract
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Non-coding RNAs (ncRNA) are involved in essential biological processes in all three domains of life. The regulatory potential of ncRNAs in Archaea is, however, not fully explored. In this study, RNA-seq analyses identified a set of 29 ncRNA transcripts in the hyperthermophilic archaeon Sulfolobus acidocaldarius that were differentially expressed in response to biofilm formation. The most abundant ncRNA of this set was found to be resistant to RNase R treatment (RNase R resistant RNA, RrrR(+)) due to duplex formation with a reverse complementary RNA (RrrR(-)). The deletion of the RrrR(+) gene resulted in significantly impaired biofilm formation, while its overproduction increased biofilm yield. RrrR(+) was found to act as an antisense RNA against the mRNA of a hypothetical membrane protein. The RrrR(+) transcript was shown to be stabilized by the presence of the RrrR(-) strand in S. acidocaldarius cell extracts. The accumulation of these RrrR duplexes correlates with an apparent absence of dsRNA degrading RNase III domains in archaeal proteins.
es_ES
Patrocinador
dc.description.sponsorship
Deutsche Forschungsgemeinschaft
DFG RA 2169/3-1
Comision Nacional de Ciencia y Tecnologia de Chile
FONDECYT 1130229
Max Planck Society