Chitosan-triclosan particles modulate inflammatory signaling in gingival fibroblasts
Author
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Pavez, L.
Author
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Tobar, N.
Author
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Chacón, C.
Author
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Arancibia, R.
Author
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Martínez, C.
Author
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Tapia, C.
Author
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Pastor, A.
Author
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González, M.
Author
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Martinez, J.
Author
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Smith, P. C.
Admission date
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2018-10-08T15:17:07Z
Available date
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2018-10-08T15:17:07Z
Publication date
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2018-04
Cita de ítem
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J Periodont Res. 2018;53:232–239
es_ES
Identifier
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10.1111/jre.12510
Identifier
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https://repositorio.uchile.cl/handle/2250/152005
Abstract
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Background and Objectives: An important goal of periodontal therapy is the modulation of the inflammatory response. To this end, several pharmacological agents have been evaluated. Triclosan corresponds to an antibacterial and anti-inflammatory agent currently used in periodontal therapy. Chitosan is a natural polymer that may act as a drug delivery agent and exerts antibacterial and anti-inflammatory activities. Therefore, an association between both molecules might be useful to prevent inflammation and tissue destruction in periodontal tissues.
Material and Methods: In the present study, we have generated chitosan-triclosan particles and evaluated their morphology, charge, biocompatibility and gene expression analysis in human gingival fibroblasts.
Results: The chitosan-triclosan particles size and Z potential were 129 +/- 47 nm and 51 +/- 17 mV respectively. Human gingival fibroblast viability was not affected by chitosan-triclosan. A total of 1533 genes were upregulated by interleukin (IL)-1 beta. On the other hand, 943 were downregulated in fibroblasts stimulated with IL-1 beta plus chitosan-triclosan particles. Fifty-one genes were identified as molecular targets upregulated by IL-1 beta and downregulated by the chitosan-triclosan particles. The gene ontology analysis revealed that these genes were enriched in categories related to biological processes, molecular function and cellular components. Furthermore, using real-time reverse transcription-polymerase chain reaction beta-actin, fibronectin, inter-leukin-6 and IL-1b genes were confirmed as targets upregulated by IL-1 beta and downregulated by chitosan-triclosan particles.
Conclusion: Our results show that chitosan-triclosan particles are able to modulate the inflammatory response in gingival fibroblasts. This effect might be useful in the prevention and/or treatment of inflammation in periodontal diseases.
es_ES
Patrocinador
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Chilean Fund for Science and Development FONDEF
DO8I1141
Colgate-Palmolive Chile